Cytotoxicity effect assessment of acid purified carbon nanotubes modified with cetyltrimethyl ammonium bromide  

十六烷基三甲基溴化铵修饰的羧基化单壁碳纳米管的细胞毒性(英文)

作　　者：甘丽[1] 阎雪彬[1,2] 杨金凤[3] 谷永红[1] 黄东[1,4] 章饶香[1] 黄利华[1] 

机构地区：[1]中南大学湘雅三医院,长沙410013 [2]中南大学粉末冶金国家重点实验室,长沙410083 [3]中南大学湘雅医学院附属肿瘤医院,长沙410013 [4]中南大学疼痛医学研究所,长沙410013

出　　处：《Transactions of Nonferrous Metals Society of China》2013年第11期3346-3352,共7页中国有色金属学报（英文版）

基　　金：Project(81172546)supported by the National Natural Science Foundation of China;Project(20120162110078)supported by Doctoral Fund of Ministry of Education of China;Project(2011ssxp198)supported by the Graduate Degree Thesis Innovation Foundation of Central South University,China;Project(13JJ2015)supported by Hunan Provincial Natural Science Foundation of China

摘　　要：The cytotoxicities of single-walled carbon nanotubes （SWNTs） and acid purified single-walled carbon nanotubes （SWNT-COOH） were investigated by spectroscopic analysis. Cell viability and cell apoptosis were applied to assessing the cytotoxicity of SWNT-COOH, cetyltrimethyl ammonium bromide （CTAB） and acid purified carbon nanotubes modified with cetyltrimethyl ammonium bromide （SWNT-COOH/CTAB）. The results indicate that SWNTs are more toxic than SWNT-COOH. Concentration and time-curve analyses indicate that cytotoxicity of SWNT-COOH/CTAB is more related to the toxicity of the surfactant CTAB. The cytotoxicity effect of CTAB and SWNT-COOH/CTAB is acceptable at low concentrations （0.5-25μg/mL）. The cytotoxicity observation suggests that SWNT-COOH/CTAB can safely applied to biomedical field at low concentrations （0.5-25μg/mL）.采用十六烷基三甲基溴化铵（CTAB）作为表面活性剂修饰羧基化的单壁碳纳米管（SWNT-COOH/CTAB），并对原始单壁碳纳米管（SWNTs）与羧基化修饰的单壁碳纳米管（SWNT-COOH）进行材料学特征比较。通过细胞活力和细胞凋亡实验对SWNTs、SWNT-COOH和SWNT-COOH/CTAB的细胞毒性进行比较。结果表明，羧基化修饰的单壁碳纳米管比原始单壁碳纳米管的毒性小，单壁碳纳米管经羧基化后其毒性降低；浓度及时间曲线显示SWNT-COOH/CTAB的毒性与表面活性剂CTAB相关，CTAB和 SWNT-COOH/CTAB的细胞毒性在低浓度范围内（0.5-25μg/mL）是可接受的。十六烷基三甲基溴化铵修饰的羧基化单壁碳纳米管在低浓度范围（0.5-25μg/mL）内可以较安全地用于生物医学领域。

关 键 词：single-walled carbon nanotube cetyltrimethyl ammonium bromide CYTOTOXICITY acid purification APOPTOSIS 

分 类 号：TB383.1[一般工业技术—材料科学与工程] O613.71[理学—无机化学]