皮下免疫热休克蛋白65对高密度脂蛋白抗炎抗氧化功能的影响  

Effect of Heat Shock Protein-65 on the Anti-oxidant and Anti-inflammatory Proper-ties of High Density Lipoprotein

作　　者：孙海阁[1] 田迪[1] 谭迎[1] 刘挺榕[1] 罗甜甜[1] 赖文岩[1] 郭志刚[1] 

机构地区：[1]南方医科大学南方医院心内科,广东省广州市510515

出　　处：《中国动脉硬化杂志》2013年第11期977-981,共5页Chinese Journal of Arteriosclerosis

基　　金：广东省科技计划项目(2011B031800065)

摘　　要：目的观察不同剂量热休克蛋白65对载脂蛋白E基因敲除小鼠高密度脂蛋白抗炎抗氧化功能的影响。方法 8周龄载脂蛋白E基因敲除小鼠随机分为磷酸盐缓冲液对照组、5μg热休克蛋白65组及25μg热休克蛋白65组,分别于第3、6周进行皮下免疫。第16周取标本,分别检测血脂水平、血清屏氧酶1活性、髓过氧化物酶活性、高密度脂蛋白炎症指数及炎症因子白细胞介素10和干扰素γ含量。结果随着热休克蛋白65剂量的增加,血清高密度脂蛋白胆固醇水平和白细胞介素10表达量逐渐下降,屏氧酶1活性逐渐降低,高密度脂蛋白炎症指数、髓过氧化物酶活性和干扰素γ表达量则逐渐升高。与对照组比较,25μg热休克蛋白65组血清高密度脂蛋白胆固醇水平和白细胞介素10表达量明显下降(P<0.01),屏氧酶1活性明显降低(P<0.05),高密度脂蛋白炎症指数、髓过氧化物酶活性、干扰素γ表达量则显著升高(P<0.01)。结论热休克蛋白65可引起炎症反应,损害高密度脂蛋白抗炎抗氧化功能,并且这种作用与其剂量有关。Aim To determine the effect of heat shock protein-65 （HSP65） on the anti-oxidant and anti-inflamma- tory properties of high density lipoprotein （HDL） in apolipoprotein E gene knocked-out （ApoE -/- ） mice. Methods Eight-week-old ApoE-/- mice were randomly divided into three groups： phosphate buffer as control group, 5 μg HSP65 group and 25 μg HSP65 group. The mice were boosted twice under the same protocol following three and six weeks. Sixteen weeks after the first immunization, serum lipids, paraoxonase-1 （PON-1） activity, myeloperoxidase （MPO） activity, high density lipeprotein inflammatory index （HII）, the level of cytokines interleukin-10 （IL-10） and interferon-~ （IFN-~/） were assessed. Results With the increasing dose of HSP65, HDLC level, IL-10 expression and PON-1 activity reduced grad- ually, whereas HII, MPO activity, and the IFN-~ expression elevated. Compared with control group, markedly decreased HDLC, IL-10 level and PON-1 activity but a significant enhancement of the serum HII, MPO activity, and IFN-levels were resulted in 25 μg HSP65 group （ all P 〈 0. 05）. Conclusion HSP65 could induce inflammatory response and impair the anti-oxidant and anti-inflammatory properties of HDL, which was related to the dose of HSP65.

关 键 词：热休克蛋白65 高密度脂蛋白 抗炎 抗氧化 

分 类 号：R363[医药卫生—病理学]