山东汉族人群19个STR基因座的遗传多态性  

Genetic Polymorphisms of 19 STR Loci in Shandong Han Population

作　　者：张茂修[1,2] 韩淑毅[1,2] 高洪梅[1,2] 孙善会[1,2] 肖东杰[1,2] 刘洋[1,2] 汪运山[1,2] 

机构地区：[1]山东大学附属济南市中心医院,山东济南250013 [2]济南迪恩法医司法鉴定所,山东济南250013

出　　处：《法医学杂志》2013年第6期440-443,446,共5页Journal of Forensic Medicine

摘　　要：目的调查山东汉族人群19个STR基因座的群体遗传学资料,为亲权鉴定提供遗传学数据。方法采用GoldeneyeTM20A试剂盒对山东汉族人群中205例无关个体进行基因分型,得到19个STR基因座的等位基因频率及群体遗传学参数。对IdentifilerTM、SinoFilerTM、PowerPlex誖16和GoldeneyeTM20A 4个试剂盒进行比较,并对1例特殊的亲子鉴定案件进行分析。结果获得山东汉族人群19个STR基因座的群体遗传学参数。累积个体识别率(CDP)及累积非父排除率(CPE)从高到低分别为GoldeneyeTM20A、SinoFilerTM、PowerPlex誖16、IdentifilerTM试剂盒。对实际案件进行分析,作为二联体,IdentifilerTM试剂盒无被排除基因座,而SinoFilerTM、PowerPlex誖16和GoldeneyeTM20A试剂盒均不能排除父子关系。结论与IdentifilerTM、SinoFilerTM和PowerPlex誖16 3种试剂盒进行比较,GoldeneyeTM20A试剂盒效能更高,但不能完全满足二联体鉴定的要求。Objective To investigate the genetic polymorphisms of 19 STR Loci in Shandong Han popu- lation in order to provide the genetic data for paternity testing. Methods The genotypes of 205 unrelated individuals in Shandong Hart population were typed by GoldeneyeTM 20A kit to get the allele frequencies and population genetic parameters of 19 STR loci. Four kits, IdentifilerTM kit, SinoFilerTM kit, PowerPlex 16 kit, and GoldeneyeTM 20A kit, were compared with each other and used in the analysis of a special paternity test case. Results The population genetic parameters of 19 STR loci in Shandong Han Popula- tion were obtained. The cumulative discrimination power （CDP） and cumulative probability of exclusion （CPE） ranked from high to low were GoldeneyeTM 20A kit, SinoFilerTM kit, PowerPlex 16 kit and Identi- filerTM kit, respectively. As duo case, the result of the real case showed that IdentifilerTM kit had no ex- cluding loci, and none of the SinoFilerTM kit, PowerPlex 16 kit or GoldeneyeTM 20A kit could exclude fatherhood. Conclusion Compared with IdentifilerTM kit, SinoFilerTM kit, and PowerPlex 16 kit, Gold- eneyeTM 20A kit shows the higher efficiency than the others, but is not completely satisfied for duo cases.

关 键 词：法医遗传学 短串联重复序列 多态现象 遗传 山东 汉族 

分 类 号：R394[医药卫生—医学遗传学]