癫痫大鼠脑内caveolin-1的表达  

Study on caveolin-1 expression in the brain of epileptic rats

作　　者：徐静[1] 连永亮[1] 于德钦[1] 戴淑芳[1] 宫德正[1] 殷盛明[1] 孙艺平[1] 

机构地区：[1]大连医科大学机能学实验室,辽宁大连116044

出　　处：《中风与神经疾病杂志》2013年第12期1096-1097,共2页Journal of Apoplexy and Nervous Diseases

基　　金：辽宁省教育厅高等学校重点实验室项目(LS2010057)

摘　　要：目的探讨癫痫大鼠脑内caveolin-1（Cav-1）的表达及分布。方法以氯化锂-匹罗卡品诱发大鼠癫痫发作后1～15d，通过Westernblot及免疫组织化学法检测大鼠脑内Cav-1蛋白的表达。结果大鼠癫痫发作后1d时，海马内Car-1蛋白的表达明显升高，4d时达到最高水平，7d时下降至正常水平；在海马的CA，区、CA，区、齿状回及皮层中呈棕黄色的Cav-1阳性染色颗粒明显增多。结论癫痫发作后1-7d可引起大鼠海马及皮层中Car-1表达增加。Objective To explore the expression of caveolin-1 （ Car-1 ） in the brain of the epileptic rats. Methods The rats were induced seizure by pilocarpine. Then the expression of Cav-1 protein in the brain were detected by Western blot and immunohistochemistry. Results The expression of Cav-1 in CAI , CA3 and the dentate gyrus（DG） of hippocampus and cortex were higher than normal rats at I d after rats status epilepticus. The highest level of Car-1 expression was at 4d, and it decreased to normal level at 7d. Conclusion These results suggest that the expression of Cav-1 was increased in the hippocampus and cortex at 1 -7d on epilepsy＇ s rats.

关 键 词：癫痫 CAVEOLIN-1 海马 

分 类 号：R742.1[医药卫生—神经病学与精神病学]