慢性吸入二氧化氮诱发大鼠DNA-蛋白质交联和微核效应  

DNA-protein Crosslinks and Micronuclei Induced by Chronic Nitrogen Dioxide Inhalation in Rats

作　　者：韩明[1] 李广科[1] 桑楠[1] 

机构地区：[1]山西大学环境与资源学院,环境科学研究所,太原030006

出　　处：《应用与环境生物学报》2013年第6期941-944,共4页Chinese Journal of Applied and Environmental Biology

基　　金：高等学校博士学科点专项科研基金项目(20121401110003);山西省留学基金项目(2011-013;2012-009);山西省高校中青年拔尖创新人才支持计划(20120201)资助~~

摘　　要：为了探讨二氧化氮(NO2)慢性、低浓度暴露对机体的遗传损伤作用,通过建立整体动物动式染毒模型,以不同浓度NO2(0.25、5 mg m-3)对大鼠进行慢性熏气染毒处理,考察了大鼠不同脏器(脑、肺、肝、肾、脾、心)DNA-蛋白质交联系数及骨髓微核发生率的变化.结果表明,NO2慢性暴露可导致大鼠不同脏器DNA-蛋白质交联度升高,诱导骨髓微核发生率增加,存在明显的浓度-效应关系,高浓度时表现更为明显,与对照组比较具有统计学差异(P<0.05),这可能是其慢性、低浓度暴露引发健康危害的一个重要原因.Nitrogen dioxide （NO2） is a ubiquitous reactive free-radical gas associated with momentary and chronic health effects. In the present study, chronic NO2？inhalation-induced effects on the DNA-protein crosslinks （DPC） and micronuclei （MN） in rats were investigated, by exposing Wistar rats to NO2？at 0.25 and 5？mg m-3. The results showed that chronic inhalation exposure of rats to NO2 not only induced the formation of DPC in cells of various internal organs （the brain, lungs, liver, spleen, kidneys and heart）, but also resulted in obvious increase of MN frequency in the bone marrow cells of rats. Furthermore, the above responses showed significant linear dose-dependent manners. These results implicated that NO2 is a clastogenic and genotoxic agent, which may be an important reason for health problem induced by chronic inhalation exposure to NO2.

关 键 词：NO2 大鼠 DNA-蛋白质交联 微核 

分 类 号：X174[环境科学与工程—环境科学]