Colorimetric Determination of Monosodium Glutamate in Food Samples Using Colorimetric Determination of Monosodium Glutamate in Food Samples Using L-glutamate Oxidase-glutamate Oxidase  

作　　者：Afraa Alnokkari Mounir Ataie Zaid Alasaf 

机构地区：[1]Department of Analytical and Food Chemistry,Faculty of Pharmacy,University of Damascus

出　　处：《应用与环境生物学报》2013年第6期1069-1072,共4页Chinese Journal of Applied and Environmental Biology

摘　　要：A rapid, sensitive and simple chromogenic method was developed for quantitative determination of monosodium glutamate(MSG) in food samples. The method incorporated a glutamate oxidase and peroxidase. The librated H 2 O 2 from a glutamate sample as a result of enzymatic action was measured using 4-aminoantipyrine and phenol as a chromogenic reagent at 502 nm. Glutamate calibration curve was linear up to 125 mmol L-1 with a detection limit of 2 mmol L-1. The results of analyzing L-glutamate in various food samples using the approach were compared to those of a standard procedure employing HPLC method. MSG concentration in different samples ranged from 0.93 to 4.9 g/kg. This method is relatively sensitive and specifi c, without the need of pre-treatment for sample. Fig 5, Tab 1, RefA rapid, sensitive and simple chromogenic method was developed for quantitative determination of monosodium glutamate （MSG） in food samples. The method incorporated a glutamate oxidase and peroxidase. The librated H202 from a glutamate sample as a result of enzymatic action was measured using 4-aminoantipyrine and phenol as a chromogenic reagent at 502 nm. Glutamate calibration curve was linear up to 125 mmol L~ with a detection limit of 2 mmol L~. The results of analyzing L-glutamate in various food samples using the approach were compared to those of a standard procedure employing HPLC method. MSG concentration in different samples ranged from 0.93 to 4.9 g/kg. This method is relatively sensitive and specific, without the need ofpre-treatment for sample.

关 键 词：《应用与环境生物学报》 期刊 编辑部 编辑工作 读者 

分 类 号：TS207.3[轻工技术与工程—食品科学]