LAMP法检测转基因大豆A2704-12品系  

Detection of Genetically Modified Soybean Line A2704-12 in Soybean and Its Products by LAMP Method

作　　者：邵碧英[1] 陈文炳[1] 曾莹[1,2] 陈彬[1] 郑晶[1] 缪婷玉[1] 彭娟[1] 

机构地区：[1]福建出入境检验检疫局,福建福州350001 [2]福建农林大学食品科学学院,福建福州350002

出　　处：《食品科学》2013年第24期202-207,共6页Food Science

基　　金：福建出入境检验检疫局科技计划项目(FK2012-26);出入境检验检疫行业标准计划项目(2011B286k)

摘　　要：根据转基因大豆A2704-12品系的特异序列设计了4套环介导等温扩增(LAMP)引物。通过反应温度优化、特异性测定,筛选出1套特异性强的LAMP引物。对筛选到的LAMP引物进行反应条件、反应体系的优化,建立了转基因大豆A2704-12品系的LAMP检测方法。结果表明:建立的LAMP检测方法能特异、稳定地检测转基因大豆A2704-12品系,检测限达到0.1%(m/m)。应用建立的LAMP方法检测大豆及其制品中转基因大豆A2704-12品系,检测结果与实时荧光聚合酶链式反应(PCR)结果完全一致。According to the specific sequence of genetically modified soybean line A2704-12, four sets of primers for loop- mediated isothermal amplification （LAMP） were designed. One set of highly specific primers was screened through optimizing the reaction temperature and determining the specificity. An LAMP method for the detection of genetically modified soybean line A2704-12 was developed after optimizing the reaction conditions and system. The specificity, sensitivity and stability of the LAMP method were evaluated. The results showed that the LAMP method could detect specifically and stably transgenic soybean A2704-12 strain with a low detection limit of 0.1%. The results of detection the A2704-12 strain in soybean and its products by the LAMP method were same as those from the real-time fluorescent PCR method.

关 键 词：环介导等温扩增 转基因大豆A2704 12品系 特异性检测 

分 类 号：Q78[生物学—分子生物学]