RNA干涉技术在宫颈癌相关HPV及RARβ2研究中的应用  

The application of RNA interference on study of HPV and RARβ_2 in cervical carncer

作　　者：蔡钰峰 张忠明[1] 郑鹏生[1] 刘秋霞[1] 刘莹[1] 

机构地区：[1]西安交通大学医学院第一附属医院妇产科,陕西西安710061

出　　处：《中国妇幼健康研究》2013年第6期972-974,共3页Chinese Journal of Woman and Child Health Research

基　　金：国家自然科学基金资助项目(30872741)

摘　　要：宫颈癌是最常见的女性生殖系统恶性肿瘤,其发生与发展是多因素共同参与的结果。除高危型人乳头瘤病毒(HPV)感染与宫颈癌的关系密切外,肿瘤抑制基因的表达沉默是十分重要的参与因素,且多为表观调节引起。其中维甲酸受体β2(RARβ2)的表达沉默相当普遍,但上述二者致病的关系不明。RNA干扰(RNAi)是近年来发展起来的一门新兴的在转录水平上的基因阻断技术,可以通过在细胞内引入设计的外源双链RNA诱导细胞内特定基因的沉默,从而观察相关生物因子的变化。该文就在有关宫颈癌细胞系中通过RNAi干扰HPV表达观察RARβ2表达及其表观遗传学改变的最新结果进行综述,旨在了解HPV与RARβ2之间的关系,进一步探索对宫颈癌发生机制的认识。Cervical cancer is the commonest therioma of female genital system, the occurrence and development of which result from muhifactors. Besides high-risk human papillomavirus （HPV） infection closely related with cervical cancer, the silence of tumor suppressors is also an important tactor for cervical carcinogenesis. The silence of retinoic acid receptor β2 （ RARβ2 ） is rather common among those factors, but it is still not cleat＇ how the two factors mentioned above cause the disease. RNA interference（RNAi） is a new transcription-level gene disruption technique that is discovered and developed in recent years. It is a phenomenon and also an useful research technique caused by the silence of specific gene in the cells by inducing double stranded RNA. In this article, we reviewed the change of RARβ2 expression and the epigenetic stations after interfering HPV in the cervical cancer cell lines, aiming to find out the relationship between HPV and RARβ2 and further deepen the understanding of the pathogenesis of cervical cancer.

关 键 词：宫颈癌 人乳头瘤病毒 维甲酸受体β2 DNA甲基化 RNA干扰 

分 类 号：R730.2[医药卫生—肿瘤] R271[医药卫生—临床医学]