腹腔镜手术对子宫颈癌细胞增殖相关基因表达的影响  

Effect of laparoscopic surgery on cervical cancer cell proliferation related gene expression

作　　者：黄守国[1] 秦杰[1] 陈瑾[1] 程虹[1] 蒙秋[1] 张静[1] 王海燕[1] 

机构地区：[1]中南大学湘雅医学院附属海口医院妇产科,570208

出　　处：《中国医药》2013年第12期1758-1760,共3页China Medicine

基　　金：海南省自然科学基金资助项目（30867）

摘　　要：目的探讨腹腔镜手术对子宫颈癌细胞中原癌基因N—myc、抑癌基因Fas、肿瘤转移促进基因MTA1、转移抑制基因nm23-H1表达的影响。方法选取子宫颈癌患者的标本40例，其中腹腔镜手术及开腹手术各20例，正常宫颈组织20例，采用实时荧光定量逆转录聚合酶联反应检测20例子宫颈癌患者腹腔镜术后N—myc、Fas、MTA1、nm23-H1基因表达水平的改变，并与20例子宫颈癌开腹术后及20例腹腔镜下全子宫切除术组正常子宫颈组织相对照，分别对以上各组标本进行对比分析。结果腹腔镜术后子宫颈癌组织中N—myc、Fas、MTA1、nm23-H1基因表达量分别为0．002±0．002，0．022±0．011，0．023±0．015，0．006±0．005，开腹术后子宫颈癌组织中分别为0．006±0．002，0．008±0．003，0．070±0．029，0．002±0．002，腹腔镜下全子宫切除术后正常子宫颈组织中分别为0．001±0．000，0．024±0．007，0．013±0．007．0．013±O．006，以上各组的比较差异均有统计学意义（均P〈0．05）。结论以上肿瘤相关基因与子宫颈癌细胞增殖转移有关，腹腔镜手术刺激通过影响相关基因的表达而降低子宫颈癌细胞增殖及转移潜能，是一种安全的手术方法。Objective To study the influence of laparoscopic surgery on original cancer genes including N- myc, tumor-suppressor gene Fas, tumor metastasis gene MTA1 and metastasis-suppressor gene nm23-H1. Methods The expression of N-myc,Fas, MTA1, nm23-H1 was investigated by RT-PCR in 60 cases ; among them 20 cases of cervical carcinoma treated by laparoscopic surgery and 20 cases of cervical carcinoma had open surgery. 20 cases of normal cervical had laparoscopic surgery. Results The expression of N-myc in cervical carcinoma having laparoscopic surgery and cervical carcinoma having open surgery and cervical having laparoscopic surgery were 0. 002 ± 0. 002, 0. 006 ± 0. 002, 0. 001 ± 0. 000 ; the differences among the groups were significant （ P 〈 0.05 ）. The expression of Fas in cervical carcinoma having laparoscopic surgery and cervical carcinoma having open surgery and cervical having laparoscopic surgery was 0.022 ± 0.01,0. 008 ± 0. 003, 0. 024 ± 0.007 ; the differences among the groups were significant （P 〈 0.05）. The expression of MTA1 in cervical carcinoma having laparoscopic surgery and cervical carcinoma having open surger and cervical accept laparoscopic surgery was 0. 023 ± 0. 015, 0. 070 ± 0. 029, 0.013 ±0. 007; the differences among the groups were significant （P 〈 0.05）. The expression of nm23-H1 in cervical carcinoma having laparoscopic surgery and cervical carcinoma having open surgery and cervical having laparoscopic surgery were 0. 006 ± 0. 005, 0.002 ± 0. 002, 0. 013 ± 0.006 ; the differences among the groups were significant （ P 〈 0.05 ）. Conclusion Tumor related gene is related to cervical cancer cell proliferation and metastasis; laparoscopic surgery is a safe operation method.

关 键 词：子宫颈癌 腹腔镜手术 基因表达 增殖转移 

分 类 号：R737.33[医药卫生—肿瘤]