Hsa-miR-340对膀胱癌T24细胞增殖及凋亡的影响  

Effect of hsa-miR-340 on proliferation and apoptosis of bladder cancer T24 cells

作　　者：张博威[1] 张叶飞[1] 黄江波[2] 董尚波[2] 

机构地区：[1]太原市中心医院,030009 [2]南华大学第二临床医学院

出　　处：《山西医药杂志（上半月）》2013年第12期1342-1344,共3页Shanxi Medical Journal

摘　　要：目的探讨和研究hsa-miR-340表达上调对人膀胱癌T24细胞增殖、凋亡能力的影响。方法化学合成hsa-miR-340模拟物,瞬时转染膀胱癌T24细胞,以实时荧光定量聚合酶链反应(RT-PCR)检测hsamiR-340在膀胱癌T24细胞中相对表达量,确定能够转染成功并有较高的转染率后,分别通过四甲基偶氮唑蓝(MTT)法和流式细胞计数仪,分析转染hsa-miR-340模拟物前后对膀胱癌T24细胞的增殖及凋亡能力的影响。结果①hsa-miR-340模拟物可在膀胱癌T24细胞中表达,并以RT-PCR验证相对含量较高,差异有统计学意义(P<0.05)。②增殖实验中分别在48h和72h时把实验组与阴性对照组和空白对照组相对比,膀胱癌T24细胞生长增殖明显受抑制且差异有统计学意义(P<0.05),说明hsa-miR-340过表达后,能使T24细胞增殖能力受到有效抑制。③hsa-miR-340模拟物转染T24细胞48h后,以流式细胞仪检测T24细胞凋亡率,分别为实验组(11.37±0.41)%、阴性对照组(6.72±0.32)%、空白对照组(6.62±0.23)%,比较后差异有统计学意义(P<0.05),说明hsa-miR-340过表达使T24细胞的凋亡显著增加。结论 hsa-miR-340可能参与膀胱癌的进展。Objective To investigate effects of upregulated hsa-miR-340 on the proliferation and apoptosis of bladder cancer T24 cells. Methods Hsa-miR-340 mimics were chemically synthetized and transiently transfect- ed into bladder cancer T24 cells. The expression of hsa-miR-340 in T24 cells was determined using quantitative polymerase chain reaction （RT-PCR）. Make sure that the T24 cells had been successfully transfected and on a high rate. Proliferation and apoptosis of the T24 cells were respectively evaluated using flow cytometry （FCM） and MTT assay. Results OThe bladder cancer T24 cells could be transfected by hsa-miR-340 mimics and hsa- miR-340 quantification showed high expression in T24 cells （P^0.05）. QCompared with the control group and the blank group, the experimental group could significantly inhibit proliferation of T24 cells （P〈0.05）. In the FCM test, we put each group into the flow cytometric and found that the apoptosis rates of the experimental group, the control group and the blank group were （11.37：k0.41）%,（6.72~0.32）% and （6.62＋0.23）%, re- spectively. The apoptosis of T24 cells was obviously increased （P〈0.05）. Conclusion The relative high expres- sion of hsa-miR-340 may be involved in the progression of bladder cancer.

关 键 词：膀胱肿瘤 微小RNAS 转染 细胞凋亡 

分 类 号：R737.14[医药卫生—肿瘤]