大蒜素对脂多糖诱导的大鼠肺组织水通道蛋白-1表达的影响  

Effect of Allicin on Expression of AQP-1 in Pulmonary Tissues of Septic Rats

作　　者：陈华文[1] 冯俊[1] 易旻晓[2] 祝伟[1] 李树生[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院,湖北武汉430030 [2]华中科技大学同济医学院第二临床学院,湖北武汉430030

出　　处：《中国中医急症》2013年第12期1999-2000,2021,共3页Journal of Emergency in Traditional Chinese Medicine

基　　金：湖北省自然科学基金(2011CDB196)

摘　　要：目的研究大蒜素对脂多糖(LPS)诱导的急性肺损伤(ALI)大鼠肺组织中水通道蛋白(AQP)-1表达的变化,探讨AQP-1表达与ALI的关系。方法 30只SD大鼠随机分为LPS组、大蒜素组和对照组,每组10只,在用药后6 h检测肺组织AQP-1表达情况。结果与对照组比较,LPS组AQP-1蛋白和mRNA表达明显降低,大蒜素干预后AQP-1蛋白和mRNA表达明显上升。结论大蒜素可能通过抑制LPS诱导的大鼠肺组织AQP-1表达的降低达到改善ALI的作用。Objective： To study the effect of allicin on expression of AQP-1 in the pulmonary tissues of mice with acute lung injury induced by lipopolysaceharide. Methods： 30 healthy SD mice were randomly divided into 3 groups： LPS group,allicin group and control group. ALI model was induced with LPS in all the LPS groups.Re- M-time PCR was used to observe the expression changes of AQP-1. Immunohistochemical method were used to determine the changes of AQP-1 protein in the pulmonary tissues of all the animals. Results： Compared with control group,the expression of AQP-1 were decreased in LPS group （P〈0.05）. After interfered with allicin,the expression of AQP-1 were increased （P〈 0.05）. Conclusion： Alliein can improve the pulmonary injury,and it may be related to allicin can inhibit the lower expression of AQP-1 in lipopolysaccharide-induced rats

关 键 词：大蒜素 脂多糖 水通道蛋白 

分 类 号：R285.5[医药卫生—中药学]