鲍温病HPV分型和hMLH1,hMSH2蛋白的检测  

HPV Typing and Expression of hMLH1 and hMSH2 in Bowen Disease

作　　者：张跃 袁伟[2] 贾常莎[2] 

机构地区：[1]遵义市第二人民医院皮肤科,贵州遵义563099 [2]遵义医学院附属医院皮肤科,贵州遵义563099

出　　处：《中国皮肤性病学杂志》2014年第1期11-14,共4页The Chinese Journal of Dermatovenereology

基　　金：贵州省科学技术基金(黔科合J字[2005]2060号);贵州省优秀青年人才基金(省卫生厅编号:D-241)

摘　　要：目的探讨人乳头瘤病毒感染与不同部位鲍温病(Bowen)病发病的关系并检测肿瘤错配修复基因hMLH1,hMSH2蛋白在Bowen病中的表达情况。方法 20例Bowen病组织来源于2004-2011年皮肤科病理证实标本,其中肛周和外生殖器共3例,其他部位共17例,另取10例正常皮肤为对照。采用人乳头瘤病毒核酸扩增分型方法对Bowen病皮损进行基因分型检测。免疫组化法检测Bowen病组织及10例正常皮肤组织中hMLH1,hMSH2蛋白的表达。结果 20例Bowen病患者中肛周和外生殖器、其他部位共检出6例HPV阳性(30.00%),为HPV16,HPV18,HPV33型。Bowen病组织中hMLH1,hMSH2蛋白阳性表达较正常皮肤组织降低,差异有统计学意义(P<0.05);Bowen病高危型HPV感染组中hMLH1,hMSH2蛋白阳性表达低于非感染组(P<0.05)。结论 Bowen病的发生可能与HPV感染有关,Bowen病患者皮损中hMLH1,hMSH2蛋白低表达也许与其进展为肿瘤有关联。Objective To investigate the expression and significance of hMLH1 and hMSH2 protein in Bowen diseases （BD） in order to explore the role of human papillomavirus （HPV） in the pathogenesis of Bowen disease. Methods Samples from twenty cases of BD confirmed by histopathology were collected from 2004 to 2011. Three cases were perianal/genital BD. The rest were BD on other sites. Samples from 10 normal subjects were used as control. Twenty-one HPV genotypes were detected by human papillomavirus PCR typing diagnostic kit in lesional skin of BD. Immunohistochemical technique was employed to evaluate the expression of hMLH1, hMSH2 protein in lesional skin of BD and normal skin. Results Six of 20 BD （30%） were positive for HPV16, HPV18, or HPV33. The expression levels of hMLH1 and hMSH2 protein in BD were lower than that in normal（P 〈 0.05 ）. The expression levels of hMLH1 and hMSH2 protein in the infected group were lower than that in the non-infected group（P 〈0. 05 ）. Conclusion BD could be associated with HPV infection. The low levels of hMLH1 and hMSH2 expression could be associated with the development of tumor.

关 键 词：鲍温病 人乳头瘤病毒 HMLH1 HMSH2 

分 类 号：R739.5[医药卫生—肿瘤]