白藜芦醇对胰腺癌Miapaca-2细胞侵袭转移能力影响的体外观察  

Effects of resveratrol on invasion and metastasis of pancreatic cancer Miapaca-2 cells in vitro

作　　者：秦勇[1] 马清涌 党晓燕 慕为民[1] 杨睿[1] 

机构地区：[1]西安交通大学医学院:附属西安市中心医院普外2科,陕西西安710004 [2]第一附属医院肝胆外科,陕西西安710061 [3]第二附属医院急诊科,陕西西安710004

出　　处：《西安交通大学学报（医学版）》2014年第1期64-68,共5页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：陕西省科技攻关项目(No.2011K13-01-15)~~

摘　　要：目的体外观察白藜芦醇(Res)对人胰腺癌Miapaca-2细胞侵袭转移能力的影响。方法将3种不同浓度(200、100、50μmol/L)的Res分别作用于体外常规培养人胰腺癌Miapaca-2细胞,通过黏附、侵袭及迁移实验,观察Res对人胰腺癌Miapaca-2细胞侵袭转移能力的影响;同时应用实时定量聚合酶链反应(Real-time PCR)及Western blot方法观察其对侵袭转移相关因子MMP-7和MMP-9mRNA及蛋白水平表达的影响。结果 3个浓度组Res对人胰腺癌Miapaca-2细胞的粘附、侵袭及迁移呈现明显抑制作用,呈浓度效应关系,与对照组相比,差异具有统计学意义(P<0.01);3个浓度组Res对人胰腺癌Miapaca-2细胞MMP-7和MMP-9蛋白及mRNA表达水平明显低于阴性对照组,差异有统计学意义(P<0.01),并呈浓度效应关系。结论 Res在体外可明显抑制胰腺癌Miapaca-2细胞的侵袭转移,其抑制能力与药物浓度呈正相关,作用机制可能与下调侵袭转移相关基因MMP-7和MMP-9的表达有关。Objective To investigate the effects of resveratrol on invasion and metastasis of pancreatic cancer Miapaca-2 cells in vitro. Methods The routinely cultured human pancreatic cancer Miapaca-2 cells in vitro were treated with resveratrol of three different concentrations （200, 100 and 50 μmol/L）. Then the effects of resveratrol on invasion and metastasis of pancreatic cancer Miapaca-2 cells in vitro were detected by adhesion, invasion and migration assays. The expressions of MMP-7 and MMP-9 at the protein and mRNA levels in the cells were measured by Western blot and RT-PCR. Results The effects of resveratrol on adhesion, invasion and migration of pancreatic cancer Miapaca-2 cells were decreased in a concentration-dependent manner compared with those in the control group, and the difference was significant （P^0.01）. Compared with those in negative control group, the expressions of MMP-7 and MMP-9 at the protein and mRNA levels in pancreatic cancer Miapaca-2 cells treated with resveratrol of three different concentrations were decreased in a concentration-dependent manner, and the difference was significant （P〈0.01）. Conclusion Resveratrol in vitro can remarkably restrain the invasion and migration of pancreatic cancer Miapaca-2 cells in a concentration-dependent manner. The mechanism may be related to down-regulating the expressions of MMP-7 and MMP-9.

关 键 词：白藜芦醇 胰腺癌 侵袭 转移 MMP-7 MMP-9 

分 类 号：R735.9[医药卫生—肿瘤]