脐带间充质干细胞对肝星状细胞活化、凋亡的调控作用研究  

Role of human umbilical cord mesenchymal stem cells in the regulation of activation and apoptosis of hepatic stellate cell

作　　者：陈黎明[1] 徐若男[1] 吕飒[1] 于双杰[1] 张政[1] 王福生[1] 

机构地区：[1]解放军302医院肝病生物治疗中心,北京100039

出　　处：《解放军医学杂志》2014年第1期11-14,共4页Medical Journal of Chinese People's Liberation Army

基　　金：国家自然科学基金(81202330)~~

摘　　要：目的观察人脐带间充质干细胞(UC-MSCs)对肝星状细胞LX-2活化和凋亡的影响,并分析其可能机制。方法体外分离、培养人UC-MSCs,将UC-MSCs与LX-2细胞按1:1和3:1比例进行混合共培养或Transwell共培养。通过流式细胞仪检测UC-MSCs对LX-2细胞活化、凋亡的影响。结果 HE、油红O、碱性磷酸酶染色及表型检测证实本实验所用UC-MSCs符合MSCs标准。LX-2细胞单独培养及与UC-MSCs混合共培养或Transwell共培养情况下,表达α-平滑肌肌动蛋白(α-SMA)的LX-2细胞比例分别为52.7%,43.9%(1:1)和34.2%(3:1),50.9%(1:1)和31.2%(3:1)。肝细胞生长因子(HGF)阻断实验未逆转UC-MSCs对LX-2细胞活化的抑制作用。与单独培养比较,LX-2细胞与UC-MSCs以1:1和1:3比例在Transwell共培养情况下,表达Annexin V单阳性的LX-2细胞比例由7.1%增加至14.9%和12.8%(P<0.01)。结论人UCMSCs可以抑制肝星状细胞系的活化并促进其凋亡,其抑制活化和促进凋亡不直接依赖于细胞间的相互接触,而是以细胞因子分泌的方式发生。Objective To evaluate the role of human umbilical cord derived mesenchymal stem cells （UC-MSCs） in the regulation of activation and apoptosis of hepatic stellate cells （LX-2 cells）, and analyze its possible mechanisms. Methods The UC-MSCs were isolated and co-cultured with LX-2 cells with the ratio of 1：1 or 3：1 （co-cultured or by Transwell）. The effects of UC- MSCs on the activation and apoptosis of LX-2 cells were analyzed by FACS analysis. Results HE, oil red O, alkaline phosphatase staining and phenotype detection showed that the UC-MSCs in our work met the standards of MSCs. In vitro, the ratio of LX-2 cells that expressed c^-SMA was 52.7%, 43.9%（1：1） and 34.2%（3：1）, 50.9% （1：1） and 31.2%（3：1） respectively, when LX-2 cells were cultured alone, co-cultured with UC-MSCs and cultured by Transwell. The inhibitory effect of UC-MSCs on the activation of LX-2 cells did not be reversed by hepatocyte growth factor （HGF）. When LX-2 cells co-cultured with UC-MSCs by Transwell with the ratio of 1：1 and 1：3, the ratio of Annexin V＋ LX-2 cells increased from 7.1% to 14.9% and 12.8% as compared with that when the cells were cultured alone （P〈0.01）. Conclusion Human UC-MSCs can inhibit the activation, and induce the apoptosis of hepatic stellate cells, which might be attributed to the secretion of cytokines, but not the cellular contact. Such effect can provide an important theoretical basis for the treatment of liver fibrosis by using MSCs.

关 键 词：间质干细胞 肝细胞 细胞凋亡 

分 类 号：R575.2[医药卫生—消化系统]