C57BL／6小鼠CIK细胞体外培养及表型分析  

Proliferation of Mouse CIK in Vitro and Phenotypic Analysis

作　　者：刘秋霞[1] 齐曼[1] 陈玲玲[1] 

机构地区：[1]承德市中心医院检验科,河北承德067000

出　　处：《现代检验医学杂志》2013年第6期128-129,132,共3页Journal of Modern Laboratory Medicine

摘　　要：目的体外诱导培养C57BL／6小鼠细胞因子诱导的杀伤细胞（CIK），并研究其表型。方法从脾组织中分离淋巴细胞，经过IFN-7，CD3单抗，IL-la及IL-2诱导并培养，获得大量的CIK细胞，FACS测定CIK表面CD3，CD4，CD8和NKI-1等CD分子表达情况及其百分率。结果经IFN-7，CD3单抗，IL-la和IL-2四种细胞因子诱导后，于第4天FACS测定CD3阳性细胞〉90％，CD8阳性细胞〉30％，NKI-1阳性细胞〉15％，于细胞因子诱导后第7天收获CIK细胞后FACS测定CD3阳性细胞〉87％，CD8阳性细胞〉20％和NKI-1阳性细胞〉10％。结论CIK细胞培养成功。Objective To incubate CIK cells and test its phenotype. Methods Non-adherent ficoll separated mouse spleen mononuclear cells derived from healthy C57 mouse were prepared and grown in RPMI 1640 medium. One thousand IU/ml human recombinant interferon 7 was added on day 0. After 24 hours of incubation,50 ng/ml of an anti-CD3,100 U/ml inter- leukin-la and 300 U/ml interleukin-2 were added. Cells were incubated at 37℃ in a humidified atmosphere of 5 ml/dl CO2 and sub-cultured every third day in fresh complete medium with 300 U/ml IL-2 at 33〈 106 cells/ml. CIK cells were harvested on day 7. The phenotype of CIK cells were tested by Flow cytomety using CD3, CD4,CD8 and NK1.1 on day 0, day 4 and day 7. Results The CIK cells were phenotyped with antibodies against CD3,CD8 and NK1.1 were consistent with the ex- pected results. The percentage of CD3〉90%,CD8〉30 %,NK1.1〉 10% . Conclusion The CIK cells were phenotyped with antibodies against CD3,CD8 and NK1.1 were consistent with the expected results, and percentage of CD3〉90%, CD8 30%,NK1. 1〉10%.

关 键 词：单核细胞 细胞因子诱导的杀伤细胞(CIK) 表型 

分 类 号：R-332[医药卫生]