骨桥蛋白对胰腺癌细胞金属基质蛋白酶-9转录后调控作用的研究  

Study on Effects of Osteopontin on Post-Transcriptional Regulation of MMP-9 Gene Expression in PANC-1 Cells

作　　者：周苏君[1,2] 张国强[1,2] 蒋超[1,2] 蔡海[1,2] 钟玉兵[1,2] 高兴[1,2] 王涛[1,2] 

机构地区：[1]江苏大学附属宜兴医院 [2]宜兴市人民医院普外科,宜兴市214200

出　　处：《广西医学》2013年第12期1585-1587,1590,共4页Guangxi Medical Journal

基　　金：江苏大学临床医学科技发展基金(JLY2010042)

摘　　要：目的探讨骨桥蛋白对胰腺癌细胞中金属基质蛋白酶-9转录后调控的影响。方法采用荧光定量PCR、荧光素酶报告基因技术检测骨桥蛋白对胰腺癌细胞金属基质蛋白酶-9转录后调控的影响。结果骨桥蛋白促进金属基质蛋白酶-9 mRNA半衰期的延长,上调含金属基质蛋白酶-9 3'UTR的荧光素酶报告基因的表达。结论骨桥蛋白上调胰腺癌细胞株PANC-1中金属基质蛋白酶-9转录后调控,其主要作用位点在金属基质蛋白酶-93'UTR。Objective To investigate the effects of osteopontin on post-transcriptional regulation of matrix metalloproteinase-9（ MMP-9 ） gene expression in PANC-1 cells. Methods Real-time PCR and ]uciferase reporter gene assay were used to detect the effects of osteopontin on post-transcriptional regulation of MMP-9 gene expression in PANC-1 cells. Results Osteopontin prolonged the half-life of MMP-9 mRNA and up-regulated the expression of luciferase in report gene plasmid which contained 3＇UTR of MMP-9 mRNA in PANC-1 cells. Conclusion Osteopontin activates the expression of MMP-9 partly through affecting post-transcriptional regulation of MMP-9 gene expression in PANC-1 cells, whose primary target is 3＇UTR of MMP-9.

关 键 词：胰腺癌 骨桥蛋白 金属基质蛋白酶-9 3’UTR 

分 类 号：R735.9[医药卫生—肿瘤]