HPLC法测定3种老鹳草药材中没食子酸和柯里拉京的含量  

Determination of Gallic Acid and Corilagin in 3 Species of Lao-Guan-Cao by HPLC

作　　者：许敏[1] 陈颖[1] 段素敏 刘丽芳[1] 

机构地区：[1]中国药科大学天然药物活性组分与药效国家重点实验室,江苏南京210009

出　　处：《中国野生植物资源》2013年第6期39-41,44,共4页Chinese Wild Plant Resources

基　　金：国家药典委员会2010版一部标准研究课题(YS-229;YS-230)

摘　　要：目的:建立老鹳草、野老鹳草、牻牛儿苗中没食子酸、柯里拉京含量测定方法。方法:采用HPLC法,色谱条件:色谱柱为Diamonsil C18(2)色谱柱(150 mm×4.6 mm,5μm);流动相为乙腈-0.1%磷酸梯度洗脱;流速为1.0 mL/min;检测波长为274 nm;柱温为25℃。结果:没食子酸和柯里拉京的线性范围分别是:0.202 4μg^2.024 0μg(r=0.999 6)、0.126 0~2.520 0μg(r=0.999 2),平均回收率分别是97.4%(RSD=1.4%)、96.7%(RSD=0.9%)。结论:本法简便、准确、重复性好,可用于老鹳草药材的质量评价。Aim： To establish a method for determining gallic acid and corilagin in Geranium wilfordii Maxim. , Geranium carolinianum L. and Erodium stephanianum Willd. Methods： An HPLC method was performed on a Diamonsil Cls column （150 mm ×4.6 mm, 5 m） with acetonitrile -0.1% H3PQ as mo- bile phase. The flow rate was 1.0 mL/min,the detection wavelength was set at 274 nm and the column temperature was 25 ℃. Results： The calibration curve of gallic acid and corilagin were linear in the range of 0.202 4 - 2. 024 0 g （ r = 0. 999 6 ） and 0.126 0 - 2. 520 0 μg （ r = 0. 999 2）, respectively. The average recovery of gallic acid and corilagin were 97.4% （ RSD = 1.4% ） and 96.7% （ RSD =0.9% ）, respectively. Conclusion： The method is simple, accurate and suitable for the quality control for this herb.

关 键 词：HPLC 老鹳草 野老鹳草 牻牛儿苗 没食子酸 柯里拉京 

分 类 号：R284.1[医药卫生—中药学]