番鸭细小病毒套式PCR检测方法的建立及应用  

DEVELOPMENT AND APPLICATION OF NESTED PCR FOR DETECTION OF MUSCOVY DUCK PARVOVIRUS

作　　者：李林林[1] 董嘉文[1] 孙敏华[1] 袁建丰[1] 邝瑞欢[1] 胡奇林[1] 

机构地区：[1]广东省农业科学院动物卫生研究所广东省兽医公共卫生公共实验室,广州510640

出　　处：《中国动物传染病学报》2013年第6期8-11,共4页Chinese Journal of Animal Infectious Diseases

基　　金：广东省自然科学基金博士启动项目(S2011040001867);公益性行业(农业)科研专项(201003012)

摘　　要：通过比对分析GenBank上登录的多株番鸭细小病毒(Muscovy duck parvovirus,MDPV)和鹅细小病毒(Goose parvovirus)全基因序列,选取两种病毒间相似性较低的区域设计合成了2对特异性引物,建立了能快速鉴别诊断MDPV的套式PCR方法。该法仅能特异地扩增MDPV,敏感性达到62 copies/μL,比普通PCR高1000倍。运用该方法对分离的12份临床样品进行检测,结果检测出2份MDPV。本试验所建立的套式PCR方法可用于MDPV感染的临床诊断和流行病学调查。According to the genome sequences of MDPV and GPV deposited in GenBank, two pairs of nested PCR primers were designed using the primer premier 5.0 and used for the development of a nested PCR. The nested PCR developed in the present study was 1,000 times more sensitive than a conventional PCR and specifically amplified MDPV with the sensibility of 62 copies/uL. Two out of 12 clinical duck samples were tested MDPV positive in this method. This nested PCR might be a useful method for clinical diagnosis and epidemiology of MDPD.

关 键 词：番鸭细小病毒 套式PCR 特异性 检测 

分 类 号：S858.322.659.2[农业科学—临床兽医学]