响应曲面法优化酸奶中嗜热链球菌H1的生长条件  

Optimization of growth condition for S.thermophilus H1 by response surface methodology

作　　者：王玉芹[1] 周晶[1] 沙未来[1] 

机构地区：[1]曲阜师范大学生命科学学院,山东曲阜273165

出　　处：《山东理工大学学报（自然科学版）》2013年第6期26-31,共6页Journal of Shandong University of Technology:Natural Science Edition

基　　金：国家级大学生创新创业训练计划项目(201210446007);山东省自然科学基金资助项目(ZR2010CL014);山东省高等学校科技计划项目(J12LE02);曲阜师范大学青年基金资助项目(XJ201107;XJ201001)

摘　　要：采用单因素试验和响应曲面法对酸奶中嗜热链球菌H1的最适生长条件进行了研究和优化.根据回归分析得到的最适培养条件为:温度41.02℃,pH5.74,接种量0.31mL·50mL-1,盐浓度0.23%,此时菌株的光密度OD600为1.176.经实验验证,在该条件下菌株光密度OD600为1.162,其相对误差小于2%.The growth conditions of S .thermophilus H1 were studied by single factor experiment and response surface methodology . The results showed that the optimal parameters for the growth conditions as follows ：the temperature was 41 .02℃ ,the pH was value 5 .74 ,the inoculum size was 0 .31mL · 50mL -1 ,the salt concentration was 0 .23% ,and the OD600 of strain HI was 1 . 176 .Under the optimal conditions the OD600 of strain HI was 1 .162 ,The relative error was bel-lo w 2% .

关 键 词：嗜热链球菌 响应曲面法 生长条件 优化 

分 类 号：TS252.54[轻工技术与工程—农产品加工及贮藏工程] Q936[轻工技术与工程—食品科学与工程]