海南原鸡CD14 cDNA的克隆及其生物信息学分析  

Cloning and Bioinformatics Analysis of CD14 cDNA from Hainan Jungle Fowl

作　　者：荣辉[1] 张珈宁[1] 杜丽[1] 成鹰[1] 郝永昌[1] 焦寒伟[1] 贾晓晓[1] 史巧芸[1] 吴科榜[1] 胡日查[1] 王凤阳[1] 

机构地区：[1]海南大学农学院,海南省热带动物繁育与疫病研究重点实验室(筹),海口市动物基因工程重点实验室,海南海口570228

出　　处：《中国家禽》2013年第24期7-10,共4页China Poultry

基　　金：海南省重点科技项目(090111)

摘　　要：从海南原鸡外周血白细胞中提取总RNA,以总RNA为模板,利用RT-PCR技术,成功获得海南原鸡CD14全长cDNA,并对其进行较系统的生物信息学分析,内容包括序列特征分析、同源性分析、编码蛋白质理化性质及结构预测。结果表明:海南原鸡CD14的CDS序列长度为1398 bp,编码465个氨基酸,该基因编码的蛋白质相对分子质量约为4.971 ku,理论等电点为6.41。对编码蛋白的分子结构预测发现,该蛋白为跨膜蛋白,其二级结构中,α-螺旋占47.31%,β-折叠占6.88%,无规则卷曲占45.81%。比对分析表明,海南原鸡CD14基因的核酸序列与人、小鼠、牛、猕猴的同源性均在30%～38%之间。Total RNA was extracted from peripheral blood leukocytes of Hainan jungle fowl. CD14 cDNA of Hainan jungle fowl was amplified by RT-PCR technology and the sequence was analyzed, including sequence features analysis, homology analysis and structure prediction. The bioinformatics analysis results indicated that ORF of CD14 cDNA was 1 398 bp encoding 465 amino acids. The protein relative molecular mass was about 4.971 ku,pI was 6.41. The protein was transmembrane protein,which contained 47.31% a-helix, 6.88% 13-meander, 45.81% random coil. The nucleotide sequence homology was between 30% to 38% with Homo sapiens,Mus musculus,Bos taurus,Macaca mulatta.

关 键 词：原鸡 CD14 CDNA 克隆 生物信息学分析 

分 类 号：Q811.4[生物学—生物工程]