单核细胞增生性李斯特菌与伊万诺夫李斯特菌菌体蛋白的比较蛋白质组学研究  

Proteomic Analysis of Whole Cell Lysate Proteins from Listeria monocytogenes and Listeria ivanovii

作　　者：殷月兰 张晨菊 叶舒扬 潘志明 陈祥 焦新安 

机构地区：[1]扬州大学江苏省人兽共患病重点实验室,江苏省动物主要疫病防控协同创新中心,江苏扬州225009

出　　处：《中国家禽》2013年第24期16-19,共4页China Poultry

基　　金：国家自然科学基金(31101841);江苏省科技支撑计划(BE2012367);江苏省自然科学基金(BK2011446)

摘　　要：李斯特菌属7个种中只有单核细胞增生性李斯特菌(Listeria monocytogens,LM)和伊万诺夫李斯特菌(Listeria ivanovii,LI)具有致病性。本研究对LM和LI的菌体蛋白比较蛋白质组学进行了初步研究。通过质谱鉴定,得到12个差异蛋白,功能涉及糖代谢、能量代谢、细胞周期控制以及宿主侵袭、分子伴侣等功能,为深入解析致病性李斯特菌的种间差异和遗传进化奠定了基础。Listeria genus has seven species, of which only Listeria monocytogenes （LM）and Listeria ivanovii （LI）are pathogenic. In this study,comparative proteomic analyses were conducted using the extracted proteins of bacterial cells from LM and LI. The 12 differentiated proteins were successfully obtained with matrix associated laser dissociation/ionization time of flight mass spectrometry. These proteins involved the functions related to sugar metabolism, energy metabolism, cell cycle controI and host invasion, molecular chaperones and many other events. All these results supplied the further understanding of pathogenic Listeria spp. and lay the foundation for the demonstration of genetic evolution.

关 键 词：蛋白组学 菌体蛋白 单核细胞增生性李斯特菌 伊万诺夫李斯特菌 

分 类 号：Q93[生物学—微生物学]