BCG对乳腺癌细胞增殖和凋亡的影响  

Effect of BCG on proliferation and apoptosis of human mammary cancer cells

作　　者：张瑞山[1] 李翔[1] 谢贤鑫 李欢[1] 张腾[1] 马利艳[1] 徐宏[1] 张斌[1] 

机构地区：[1]辽宁省肿瘤医院乳腺外科,辽宁沈阳110042

出　　处：《中国微生态学杂志》2013年第12期1377-1378,1382,共3页Chinese Journal of Microecology

基　　金：国家自然科学基金资助项目(81102029;81172047)

摘　　要：目的从细胞增殖和凋亡两方面观察BCG对乳腺癌细胞MDA-MB-231的抑制作用。方法用MTT法检测BCG作用后MDA-MB-231细胞的增殖能力,采用TUNEL法检测凋亡。结果 BCG能明显降低MDA-MB-231细胞的增殖代谢活性而抑制其增殖。TUNEL法染色显示BCG可显著增加凋亡细胞。结论BCG不仅能显著抑制MDA-MB-231细胞增殖,还能有效诱导其凋亡。Objective To observe the effect of BCG on proliferation and apoptosis of MDA-MB-231 cells. Methods The proliferation inhibitory effect of BCG on MDA-MB-231 cells was detected by MTT eolormetric assay; MDA-MB- 231 cell apoptosis was observed by TUNEL method. Results BCG obviously inhibited MDA-MB-231 cells prolif- eration. BCG could induce MDA-MB-231 cell apoptosis. There were few apoptosis cells in the control group. Conclusion BCG not only significantly inhibit the proliferation of MDA-MB-231 cells but also induce their apoptosis.

关 键 词：BCG 乳腺癌 增殖 凋亡 

分 类 号：R736.3[医药卫生—肿瘤]