MMP-2和TIMP-2基因多态性与胃癌发病风险的研究  

Single nucleotide polymorphisms of MMP-2 and TIMP-2 for risk of gastric cancer

作　　者：陈凤[1] 杨淑娟[1] 田智[1,2] 温圆圆[1,3] 谢瑶[1] 潘雄飞[1] Marie Loh 黄荷[1] 兰慧[1] 温莹[1] 赵志梅[1] Richie Soong 杨春霞[1] 

机构地区：[1]四川大学华西公共卫生学院(华西第四医院),四川成都610041 [2]重庆市巴南区第二人民医院,重庆401320 [3]杭州市疾病预防控制中心,浙江杭州310021 [4]Cancer Science Institute of Singapore,National University of Singapore,NUS Singapore 117604

出　　处：《现代预防医学》2014年第1期130-133,共4页Modern Preventive Medicine

摘　　要：目的探讨西南汉族人群中基质金属蛋白酶-2(matrix metallopmteinase-2,MMP-2)和金属蛋白酶组织抑制剂-2(tissue inhibitor of metalloproteinase-2,TIMP-2)基因启动子区域单核苷酸多态性与胃癌发病风险的关系。方法在美国Sequenom MassARRAY系统上利用基质辅助激光解吸电离飞行时间质谱法(MALDI-TOF MS)针对308对胃癌病例和对照分析MMP-2-1306C/T和TIMP-2-418G/C基因多态特征。同时利用自行设计的问卷收集研究对象的人口学特征和日常生活习惯。结果 MMP-2-1306C/T的CC、CT和TT基因型在病例组和对照组中的频率分别为:79.5%、19.8%、0.6%和79.8%、19.2%、1%;TIMP-2-418G/C的GG、GC和CC基因型在病例组和对照组中的频率分别为:71.4%、26.6%、2.0%和70.5%、27.8%、1.7%;MMP-2-1306C/T(P=0.936和0.984)和TIMP-2-418G/C(P=0.817和0.898)的基因型和等位基因频率分布在胃癌组与对照组间均无统计学差异(P>0.05)。同时未发现基因-基因联合作用(OR=1.004;95%CI:0.460~2.191)和基因-环境之间的交互作用。结论本研究未发现MMP-2-1306C/T和TIMP-2-418G/C多态性与胃癌发病风险有关,且两单核苷酸多态之间无基因-基因联合作用,也未发现基因与环境的交互作用。Objective To explore the association of single nueleotide polymorphisms （SNPs） in matrix metalloproteinase-2 （MMP-2） and tissue inhibitor of metallopmteinase-2 （TIMP-2） genes with the risk of gastric cancer among Han Chinese in the southwestern region of China. Methods In a 1：1 ease-contrnl design, MMP-2 -1306C/T and TIMP-2 -418G/C were sequenced for 308 pairs of gastric cancer eases and controls using MALDI-TOF MS on the Sequenom MassARRAY platform. Demographic data and lifestyle factors were collected using a self-designed questionnaire from the subjects. Results The genotype frequencies of MMP-2 -1306 CC, CT and TT were 79.5%, 19.8% and 0.6%, respectively, among the patients while 79.8%, 19.2% and 1% among the controls. For TIMP-2 -418G/C, the frequencies of CG, GC and CC were respectively 71.4%, 26.6% and 2.0% among the patients while 70. 5%, 27.8% and 1.7% among the controls. We did not observe statistically significant difference regarding allele frequencies and genotype distributions of MMP-2 -1306C/T （P=0.936 and 0.984） and TIMP-2 -418G/C （P^0.817 and 0.898） between the pa- tients and controls. No gene-gene （OR = 1.004; 95%CI, 0.460-2.191） or gene-environment interactions were noticed. Conclusion There is no association between bIMP-2 -1306C/T, TIMP-2 -418G/C and gastric cancer risk among Han Chinese. No synergic im- pact is noticed between the two SNPs for development of gastric cancer.

关 键 词：胃癌 MMP-2 TIMP-2 单核苷酸多态性 

分 类 号：R735.2[医药卫生—肿瘤]