高效液相色谱分析化妆品中的防腐剂氯苯甘醚  

Analysis of the preservative chlorphenesin in cosmetics by high performance liquid chromatography

作　　者：朱会卷[1] 张卫强[1] 杨艳伟[1] 朱英[1] 

机构地区：[1]中国疾病预防控制中心环境与健康相关产品安全所,北京100021

出　　处：《色谱》2014年第1期95-99,共5页Chinese Journal of Chromatography

基　　金：国家食品药品监督管理局保健食品化妆品监管司项目([2012]75)

摘　　要：建立了化妆品中防腐剂氯苯甘醚的高效液相色谱检测方法。采用C18色谱柱（250mm×4．6mm，5μm）和二极管阵列检测器，以1．0mL／min甲醇一水（55：45，v／v）为流动相，检测波长为280nm，柱温为25℃。方法的检出限（以3倍信噪比计）可达到3ng；在1～500mg／L范围内，峰面积与质量浓度呈良好的线性关系，相关系数为1．0000。高、中、低不同水平的加标回收率为99．0％～103％，相对标准偏差≤1．2％。同时进行了干扰试验和实际样品的测定，并组织3家实验室进行了方法验证。该方法操作简便，准确度、灵敏度和稳定性高，可用于化妆品中防腐剂氯苯甘醚的检测。An analytical method was developed for the determination of the preservative of chlorphenesin in cosmetics by high performance liquid chromatography （HPLC）. A C18 column （250 mm ×4.6 mm, 5 μm） and a photodiode array detector were used. The mobile phase was methanol-water （55： 45, v/v） with a flow rate of I. 0 mL/min. The detection wavelength was set at 280 nm and the column temperature was 25 ℃. The limit of detection was 3 ng. A good linear relationship was obtained between the peak area and the mass concentration of chlorphe- nesin in the range of 1 - 500 mg/L and the correlation coefficient was 1, 000 0. The recoveries of chlorphenesin at different spiked levels were 99.0% - 103% with the relative standard deviations （RSD） ≤ 1.2%. Interference test and sample test were also applied meanwhile and validation experiments were performed by three laboratories. The method is simple, sensitive, accurate, stable and suitable for the determination of chlorphenesin in cosmetics.

关 键 词：高效液相色谱 防腐剂 氯苯甘醚 化妆品 

分 类 号：O658[理学—分析化学]