2种不同启动子驱动下1-SST基因转化甘蔗的抗旱性比较  

Drought Resistance of the Transformed Sugarcanes with 1-SST Gene Driven by Two Different Promoters

作　　者：武媛丽[1] 张树珍[1] 李晓君[1] 蔡文伟[1] 杨本鹏[1] 

机构地区：[1]中国热带农业科学院热带生物技术研究所中国热带农业科学院甘蔗研究中心农业部热带作物生物学与遗传资源利用重点实验室,海南海口571101

出　　处：《热带作物学报》2014年第1期1-6,共6页Chinese Journal of Tropical Crops

基　　金：863重点项目(No.SS2013AA100604);中央级公益性科研院所基本科研业务费专项(No.1630052012013;No.ITBB120501)

摘　　要：将从菊芋中克隆到的蔗糖:蔗糖-1-果糖基转移酶基因(即1-SST基因),与根部特异性表达启动子pPST2a组合,通过农杆菌介导法获得转pPST2a:1-SST基因甘蔗植株,通过分子检测共得到15个阳性转基因株系。将前期工作得到的转rbcs:1-SST基因甘蔗5个株系与转pPST2a:1-SST基因甘蔗进行抗旱性比较。结果发现,转pPST2a:1-SST基因甘蔗的抗旱性高于转rbcs:1-SST基因甘蔗。与启动子rbcs相比,启动子pPST2a能够更大程度上增强转1-SST基因甘蔗植株的抗旱性。Fructan is a nonreducing polysaccharides of glucose that functions as a protectant in the stabilization of biological structures and enhances the stress tolerance under abiotic stresses in organisms. The sucrose：sucrse 1- fructosyltransferase （1-SST） gene from Jerusalem artichoke combined with root specific promoter pPST2a was transferred into sugarcane（Saccharumofftcinaram L.） using Agrobatorium-mediated method. Results indicated that 15 pPST2a：I-SST transgenic lines were successfully obtained according to molecular detection. Compared the drought resistance of the transgenic sugarcane between the one with pPST2a：1-SST lines obtained in the study and the other one with rbcs：1-SST obtained in previous research. The resuults showed that drought resistance of the transgenic sugarcane with pPST2a：1-SST lines was better than the one with rbcs：l-SST. And it revealed that promoter pPST2a could make drought resistance of transgenic sugarcane even stronger than the promoter rbcs.

关 键 词：果聚糖 1-SST 转基因甘蔗 抗旱性比较 

分 类 号：S59[农业科学—作物学] Q812[生物学—生物工程]