2种枇杷属植物的trnH-psbA条形码序列变异及其近缘属系统发育初步研究  

Sequence Variation of trnH-psbA Barcode in Two Eriobotrya Plants and Phylogenetic Relationships with Related Genera

作　　者：胡文舜[1,2] 蒋际谋[1,2] 黄爱萍[2,3] 许奇志[1,2] 陈秀萍[1,2] 郑少泉[1,2] 

机构地区：[1]福建省农业科学院果树研究所,福建福州350013 [2]福建省龙眼枇杷育种工程技术研究中心,福建福州350013 [3]福建省农业科学院农业经济与科技信息研究所,福建福州350003

出　　处：《热带作物学报》2014年第1期12-16,共5页Chinese Journal of Tropical Crops

基　　金：福建省自然科学基金项目(No.2011J01099);农业部农业科研杰出人才及其创新团队;公益性行业(农业)科研专项经费项目(No.201003073);农业部作物种质资源保护项目(No.NB2130135);农业部热带作物种质资源保护项目(No.13RZZY-15)

摘　　要：选用枇杷野生近缘种‘栎叶枇杷’和栽培种‘早钟6号’、‘香妃’．对其叶绿体基因组trnH—psbA基因间隔区进行PCR克隆与测序．利用生物信息学方法分析其序列特征．并构建苹果亚科内19个属的系统发育树。结果表明：trnH-psbA序列长323～392bp（GenBank登录号：KF022254、KF022255、KF022256），G＋C含量30．10％，33．13％，共有1个核苷酸替换（SUbstitution）和2个插入／缺失（indel）；基于trnH—psbADNA条形码序列的分子系统进化分析结果表明．苹果亚科内形成2个大的分支，枇杷属与石斑木属的亲缘关系最近（平均遗传距离为0．055）。此结果表明叶绿体基因trnH-psbA序列可有效地进行枇杷属内物种鉴别及属间分类，是枇杷属植物DNA条形码研究的标准基因之一。The chloroplast genome trnH-psbA intergenic regions of three loquat （wild relatives ＇E.prinoides＇, cultivars ＇Zaozhong No.6＇ and ＇Xiangfei＇ ）were cloned and sequenced. The sequence characteristics were analyzed using bioinformatics methods and the phylogeny evolution tree was constructed with 19 genus of Maloideae. The results showed that the length of trnH-psbA sequence was 323-392 bp（GenBank accession number： KF022254, KF022255, KF022256）, and its G＋C content was 30.10%~33.13%. A total of 1 substitution and 2 indels were obtained in the data. Molecular phylogenetic anaylised based on the sequence of trnH-psbA DNA barcode reflected the relationship between Eriobotrya and Rhaphiolepis was closer（the average genetic distance was 0.055）, and Maloideae was divided into two large branches. This paper suggested that trnH-psbA DNA barcode could be used as one of the standard gene to identify or classify plants of the Eriobotrya and its relative genus.

关 键 词：枇杷属 TRNH-PSBA DNA条形码 序列分析 系统进化 

分 类 号：S667.3[农业科学—果树学]