抑制性ODN对溃疡性结肠炎小鼠Th1/Th2免疫失衡的调节作用  

Th1/Th2 immune balance induced by inhibitory ODN in mice with ulcerative colitis

作　　者：孙鹏[1] 周晓晶[2] 刘伯岩[2] 杨文颖[1] 

机构地区：[1]吉林省人民医院消化内科,吉林长春130021 [2]长春中医药大学基础医学院生物化学与分子生物学教研室

出　　处：《中国实验诊断学》2013年第12期2133-2135,共3页Chinese Journal of Laboratory Diagnosis

基　　金：吉林省自然科学基金面上项目(201015221)

摘　　要：目的研究抑制性ODN对溃疡性结肠炎小鼠Th1/Th2免疫失衡的调节作用。方法首先利用我们已发表的研究[1]中的方法建立小鼠溃疡性结肠炎模型。建模成功后将结肠炎小鼠随机分为两组,分别给予腹腔内注射PBS、A151,记作PBS+UC组、A151+UC组,同时设一组健康小鼠对照并给予腹腔注射PBS,记作PBS+健康对照组。每组分别连续注射7天,末次注射后7天取小鼠血清并分离小鼠结肠组织制备匀浆。利用ELISA方法检测血清及结肠匀浆中IL-6、TNF-α、IL-10、IL-4水平。结果经检测发现抑制性ODN能明显下调IL-6和TNF-α的水平,而上调IL-10和IL-4水平。结论抑制性ODN对小鼠溃疡性结肠炎的抑制作用或许是通过调节Th1/Th2平衡来实现的。Objective ,To study if inhibitory ODN could induce Thl/ Th2 immune balance induced by inhibitory ODN in mice with ulcerative colitis （UC）. Methods Ulcerative colitis（UC）was induced by DSS in mice. The mice with UC were randomly divided into two groups. Mice with UC were injected with follows by i. p.. PBS,A151 ,respectively. At 7 days after last injection the mice were sacrificed. Serum and colons were taken out to undergo cytokines examina- tion. The levels of dytokines were tested by enzyme linked immunosorbent assay （ELISA）. Results A151 resulted in down-regulation of the level d IL-6 and TNF-a in serum and colonic tissue,and strikingly up-regulation of the level of IL-10 and IL-4. Conclusion The inhibitory ODN could therapy UC in mice by regulation of Tbl/Th2 immune balance.

关 键 词：溃疡性结肠炎 Th2免疫反应 Th1免疫反应 

分 类 号：R574.62[医药卫生—消化系统]