热疗对C6胶质瘤细胞自噬的影响  

Effects of hyperthermia on C6 glioma cell autophagy

作　　者：秦丽娟[1] 王艳蕾[1] 张田[1] 孙娜[1] 王银环[1] 赵喜庆[2] 

机构地区：[1]河北联合大学基础医学院生理学教研室,唐山063000 [2]河北联合大学附属唐山市人民医院神经外科,唐山063000

出　　处：《临床与实验病理学杂志》2013年第12期1320-1322,1328,共4页Chinese Journal of Clinical and Experimental Pathology

基　　金：河北省卫生厅科研基金(20100465;20110165;20120144);河北联合大学培育基金(SP201310)

摘　　要：目的探讨热疗降低胶质瘤侵袭性的作用与细胞自噬的关系。方法 C6细胞分为热疗0、30、60、120、180和240 min组。采用免疫组化SABC法检测C6细胞网格蛋白(Clathrin)的表达;Western blot技术检测自噬相关基因蛋白LC3B的表达;球体细胞培养法迁移实验检测C6细胞侵袭性改变。结果 C6胶质瘤细胞经热疗后,Clathrin表达增多,于热疗120 min时表达最多,而自噬相关基因蛋白LC3B的表达亦于热疗后120 min达高峰后开始减少,胶质瘤侵袭性于同一时间点降至最低。结论热疗可能是通过促进Clathrin的表达,引起C6细胞自噬而降低胶质瘤的侵袭性。Purpose To investigate the relationship between invasiveness inhibited by therlnotherapy and cell autophagy in glioma. Methods Cultured C6 glioma cells were randomly divided into seven groups： hyperthermia 0 min group, 30 min group, 60 min group, 120 min group, 180 min group and 240 min group. Immunohistochemistry and Western blot methods were used to dynamically detect the expression levels of Clathrin and LC3B protein in the glioma cells in each group, spheroid/wound-healing method was used to detect the changes of C6 cell invasion. Results Clathrin protein expression increased after hyperthermia treatment and reached the peak at 120 min and then gradually decreased in C6 cells, LC3B expression levels began to increase and reached a peak at 120 min and then gradually decreased in C6 cells after hyperthermia. At the same time point, glioma invasion was down to the lowest. Conclusion Hyperthermia may increase the expression of Clathrin that reduces autophagy in C6 cells and inhibits the invasiveness of glioma.

关 键 词：胶质瘤 自噬 CLATHRIN LC3B 热疗 

分 类 号：R739.41[医药卫生—肿瘤]