大豆异黄酮载药纳米颗粒对肝纤维化大鼠的抗过氧化作用  

Anti-peroxidative effects of SI-SPIO nanoparticles in liver fibrotic rats

作　　者：罗海峰[1] 杜渐[1] 孟辉[1] 巩鹏[1] 牟国煜[1] 谭广[1] 王洪江[1] 

机构地区：[1]大连医科大学附属第一医院肝胆外科,辽宁大连116011

出　　处：《大连医科大学学报》2013年第6期526-529,共4页Journal of Dalian Medical University

基　　金：大连市科委基金资助项目(2011E15SF114)

摘　　要：目的观察大豆异黄酮载药纳米颗粒对肝纤维化大鼠的抗过氧化作用。方法采用四氯化碳灌胃法建立肝纤维化大鼠模型,随机分成对照组、肝纤维化组、空白纳米颗粒组(SPIO组)、大豆异黄酮组(SI组)、载药纳米颗粒组(SI-SPIO组),每组24只。对照组给予生理盐水尾静脉注射,其他各组除四氯化碳灌胃外,分别给予生理盐水尾静脉注射、SPIO、大豆异黄酮和SI-SPIO载药纳米颗粒尾静脉注射治疗,观察对照组和治疗组之间肝脏生化指标、过氧化损伤指标和肝脏组织学变化。结果 8周后对各组大鼠的肝脏生化指标和肝纤维化程度进行评估,SI组和SI-SPIO组明显优于肝纤维化组和SPIO组;过氧化指标检测,与对照组相比,肝纤维化组和SPIO组MDA明显升高(2.45±0.77,2.69±1.01,P<0.05),GSH-Px和SOD明显降低(300.74±16.25,299.18±15.57和409.77±20.46,431.56±19.25,P<0.05);与肝纤维化组相比,SI组和SI-SPIO组MDA明显降低(0.85±0.12,0.90±0.02,P<0.05),GSH-Px和SOD明显升高(340.25±11.85,366.45±14.83和467.27±16.11,485.75±22.54,P<0.05)。SI-SPIO组过氧化指标较SI组改善更为明显(P<0.05)。结论大豆异黄酮具有抗氧化作用,能够抑制大鼠肝纤维化形成,载药纳米有助于药效的发挥。Objective To investigate the anti -peroxidative effects of SPIO nanoparticles as a drug delivery system when combined with soybean isoflavones （ SI-SPIO ） .Methods The liver fibrosis models of rats were established by CCL 4 ga-vage.120 rats were random grouped as control , fibrosis, SPIO tail vein injection, isoflavone treatment and SI -SPIO treat-ment group .Normal saline injections were given in control group , CCL4 gavage were given in other groups , as well as NS injection, SPIO, SI and SI-SPIO injections to 24 rats of each group, respectively.Biochemical and histological indicators were analyzed .Peroxidative indicators were detected as well .Results Liver fibrosis was significantly improved in SI and SI-SPIO groups vs .control both in biochemical and histological aspects .Peroxcidative indicators such as MDA increased in liver fibrotic and SPIO groups （2.45 ±0.77, 2.69 ±1.01, P〈0.05）, GSH -Px and SOD decreased （300.74 ± 16.25, 299.18 ±15.57 and 409.77 ±20.46, 431.56 ±19.25, P〈0.05）, SI administration reduced MDA （0.85 ± 0.12,0.90 ±0.02, P〈0.05）, increased GSH -Px and SOD in SI and SI -SPIO groups（340.25 ±11.85, 366.45 ± 14.83 and 467.27 ±16.11, 485.75 ±22.54, P〈0.05）.SI-SPIO group presents much more efficiency comparing with SI group （P〈0.05）.Conclusion SI has anti-peroxidative effect and inhibits liver fibrosis in rats .SPIO nanoparticles make the effects more efficient as a drug delivery system .

关 键 词：大豆异黄酮 肝纤维化 过氧化损伤 

分 类 号：R575.2[医药卫生—消化系统]