玉米赤霉烯酮对BHK细胞毒性作用的研究  

Toxic effect of zearalenone(ZEA)on BHK cells

作　　者：韩薇[1] 缪德年[2] 赵志辉[1] 

机构地区：[1]上海市农业科学院农产品质量标准与检测技术研究所,上海201403 [2]上海市农业科学院畜牧兽医研究所,上海201106

出　　处：《上海农业学报》2013年第6期44-48,共5页Acta Agriculturae Shanghai

基　　金：上海市农业科学院农产品质量标准与检测技术研究所科研基金

摘　　要：采用多种方法研究了玉米赤霉烯酮(Zearalenone,ZEA)染毒对叙利亚仓鼠肾细胞(BHK细胞)的影响。结果表明:ZEA可引起BHK细胞活力显著降低(P<0.(05),至染毒后24 h,40μg/mL ZEA剂量组细胞活力下降95%。ZEA对BHK细胞DNA的损伤作用也非常明显,40μg/mL剂量组DNA拖尾率达对照组的80倍。试验中40μg/mL剂量组24 h时检测到DNA ladder条带。ZEA使BHK细胞内SOD活性显著降低(P<0.05),细胞培养液中MDA含量则显著升高(P<0.05)。对细胞作用24 h后,最高剂量组(40μg/mL)细胞培养液中MDA含量与对照组相比升高了5倍,而细胞的抗氧化能力却降至对照组的1/4左右。上述结果表明:ZEA作用于BHK细胞后,细胞活力下降,DNA受到损伤,引发细胞凋亡;并且ZEA引发了BHK细胞过氧化物生成增加,抗氧化能力下降。The viability of BHK cells as influenced by ZEA was studied by various methods.The results showed that ZEA could significantly decreased the viability of BHK cells（P〈0.05）,and after 24 hours the viability of the cells treated with 40 μg/mL ZEA dropped by 95%.ZEA also injured the cell DNA very evidently,and the DNA tailing rate of the 40 μg/mL ZEA group was 80 times that of the control group.The DNA ladder band was detected in the experiment with 40 μg/mL ZEA for 24 h.ZEA made the cells＇ SOD activity decrease significantly（P〈0.05）and the MDA concentration in the cell culture solution increase significantly（P〈0.05）.In the group treated with 40 μg/mL ZEA for 24 h,the MDA concentration increased by 5 times over that in the control group,while the cells＇ anti-oxidation ability decreased to nearly one fourth that of the control group.The above results indicated that ZEA made the cell viability decrease and the DNA damage and finally caused the cell apoptosis,and moreover,ZEA caused the cell-produced peroxide to increase and the cells＇ antioxidation ability to decrease.

关 键 词：玉米赤霉烯酮 毒性作用 叙利亚仓鼠肾细胞 单细胞凝胶电泳 噻唑蓝 黄嘌呤氧化酶 丙二醛 

分 类 号：S858[农业科学—临床兽医学]