ETV1转录因子在胃肠间质瘤中的作用  

Role of ETV1 transcription factor in gastrointestinal stromal tumor cells

作　　者：李强[1] 徐龙[2] 刘先发[1] 

机构地区：[1]赣南医学院第一附属医院急诊科,江西省赣州市341000 [2]武汉大学中南医院消化内科,湖北省武汉市430071

出　　处：《世界华人消化杂志》2013年第35期4037-4042,共6页World Chinese Journal of Digestology

基　　金：江西省卫生厅科技计划基金资助项目;No.20081028;江西省教育厅科学技术研究基金资助项目;No.GTT09114~~

摘　　要：目的:在前期实验中,我们通过体外构建干扰人源性ETS变异基因1(human ETS variant gene1,hETV1)基因shRNA的载体,干扰ETV1表达,进而影响胃肠间质瘤细胞的侵袭作用,在此,我们继续通过上述方法,观察胃肠间质瘤细胞增殖与凋亡的影响.方法:体外构建干扰hETV1基因shRNA的慢病毒载体,感染GIST-T1细胞.实验分成3组:空白组、阴性对照组[PLVX(逆转录病毒)-shRNA组]、实验组(PLVX-shRNA-ETV1组),用Western blot方法对合成的靶点进行筛选,采用体外增殖培养试验(MTT法)和流式细胞凋亡检测感染前后细胞增殖与凋亡的情况.结果:在MTT实验的前期阶段,实验组细胞增殖数量未见明显下降,与空白组(P=0.539、P=0.188)及阴性对照组(P=0.877、P=0.992),无显著性差异,而在后期阶段,实验组细胞增殖数量明显受到抑制,与空白组(P=0.014、P=0.000、P=0.020)及阴性对照组(P=0.011、P=0.000、P=0.003),有显著性差异;同时在细胞凋亡试验中,实验组其凋亡率比空白组(P=0.000)及阴性对照组(P=0.000)明显增高,有显著性差异.结论:通过体外干扰ETV1转录因子,导致GIST-T1细胞在实验后期阶段有明显抑制作用,细胞凋亡明显增加,从而影响胃肠间质瘤细胞的进展.AIM： To investigate the role of ETV1 in the pro-liferation and apoptosis of gastrointestinal stro-mal tumor （GIST） cells. METHODS： A recombinant lentiviral vector car-rying the small interfering RNA targeting ETV1 was constructed and used to infect GIST-T1 cell. GIST cells were divided into three groups： a blank control group, a negative control group （PLVX-shRNA group）, and an experimental group （PLVX-shRNA-ETV1 group）. After infec-tion, ETV1 expression was detected by Western blot. GIST cell proliferation and apoptosis were detected by MTT assay and flow cytometry, re-spectively.RESULTS： In the early stages after lentiviral infection, cell proliferation in the experimental group showed no significant differences com-pared to the blank control group （P = 0.539, 0.188） and negative control group （P = 0.877, 0.992）. However, in the late stages, cell proliferation in the experimental group showed significant de-creases compared to the blank control group （P = 0.014, 0.000, 0.020） and negative control group （P = 0.011, 0.000, 0.003）. The rate of apoptosis in the experimental group was significantly higher compared to the blank control group （P = 0.000） and negative control group （P = 0.000）. CONCLUSION： ETV1 transcription factor may play an important role in the proliferation and apoptosis of GIST cells.

关 键 词：胃肠间质瘤 ETS变异基因1 

分 类 号：R735[医药卫生—肿瘤]