结直肠腺癌组织中CD44^+/Oct4^+癌干细胞的形态及分布  

Morphology and distribution of CD44^+/Oct4^+colorectal cancer stem cells

作　　者：张登才[1,2] 刘斌[1,2,3] 张丽华 张彩兰[1] 杨艳丽[1] 苏勤军[1] 史敏[1] 董亮[1] 哈英娣[1] 

机构地区：[1]解放军兰州军区兰州总医院病理科,甘肃省兰州市730050 [2]兰州大学基础医学院病理学研究所,甘肃省兰州市730000 [3]甘肃省干细胞与基因药物重点实验室,甘肃省兰州市730000 [4]兰州石化总医院病理科,甘肃省兰州市730060

出　　处：《中国组织工程研究》2013年第49期8461-8467,共7页Chinese Journal of Tissue Engineering Research

基　　金：甘肃省自然科学基金资助项目(096RJZA096)~~

摘　　要：背景:越来越多的证据显示CD44可作为结直肠腺癌干细胞的一个特异性的标志物,近年发现胚胎干细胞转录因子Oct4在结直肠腺癌中有表达。目的:观察CD44+/Oct4+细胞在原发性结直肠腺癌组织中的数量、位置及分布方式。方法:取108例结直肠腺癌、18例癌旁正常肠黏膜组织及18例伴不典型增生的结直肠腺瘤标本制成48点共3块组织芯片,应用免疫组织化学双重染色和苏木精-伊红染色,定位CD44+/Oct4+肿瘤细胞,观察计数并在苏木精-伊红切片的对应位置上观察其形态特征。结果与结论:免疫组织化学双重染色结果显示,癌旁正常肠黏膜中未见CD44+/Oct4+细胞,在伴不典型增生的腺瘤中可见极少量CD44+/Oct4+细胞,结直肠腺癌中可见到少量CD44+/Oct4+细胞。双阳性细胞主要分布在腺管样结构基底膜侧或共壁腺体的共壁侧,呈小灶状或散在点状分布;细胞呈卵圆形或立方状,胞浆稀少,胞核规则,均质深染,呈卵圆形或高柱状。其数量与结直肠癌分化程度呈负相关(r=-0.579,P<0.01),与肿瘤浸润深度有一定关联(r=0.236,P<0.05)。结果提示CD44+/Oct4+细胞可能是结直肠腺癌干细胞。BACKGROUND：More and more studies employ CD44 as a specific marker of colorectal cancer stem cells. Oct4 is a transcription factor of embryonic stem cells, and it has been discovered recently that there is a higher expression in primary colorectal carcinoma. OBJECTIVE：To investigate the quantity, location and distribution of CD44^＋/Oct4^＋cells in primary colorectal carcinoma. METHODS：A total y of 108 cases of human colorectal carcinoma and 18 cases of normal mucosa, 18 cases of adenoma were col ected and made into three tissue microarrays, each containing of 48 dots. The locations of CD44^＋/Oct4^＋cells were detected by double-label immunohistochemical staining and hematoxylin-eosin staining. The morphologic features of them were investigated on hematoxylin-eosin staining at the same position. RESULTS AND CONCLUSION：The results of double-label immunohistochemical staining demonstrated that there were no CD44^＋/Oct4^＋cells in normal intestine mucosa and a very smal amount of CD44^＋/Oct4^＋cells in adenoma, and double-positive cells could also be seen in colorectal carcinoma. The number of CD44^＋/Oct4^＋cells was rare and the cells were scattered or distributed focal y along the basement of gland basal side. The cells with scarce cytoplasm were square, and its nucleus was oval or high cylindrical, deeply stained and homogeneous. The quantity of CD44^＋/Oct4^＋cells was negatively correlated with the differentiation of colorectal cancer （r=-0.579, P〈0.01）, and was associated with the depth of tumor invasion （r=0.236, P〈0.05）. These findings indicate that CD44^＋/Oct4^＋cells may be colorectal cancer stem cells.

关 键 词：干细胞 结直肠腺癌 肿瘤干细胞 腺瘤 CD44^+ Oct4^+ 组织芯片 免疫组织化学双重染色 省级基金 干细胞图片文章 

分 类 号：R394.2[医药卫生—医学遗传学]