K562细胞株侧群细胞中部分白细胞分化抗原的表达  

Some leukocyte differentiation antigens express in side population cells in leukemia K562 cell line

作　　者：范瑞华[1] 郭殿选[2] 张铁成[1] 岳顺[1] 黄明德[1] 姚榕[1] 

机构地区：[1]南京医科大学附属淮安第一医院肿瘤内科,江苏省淮安市223300 [2]淮安市第二人民医院老年病科,江苏省淮安市223002

出　　处：《中国组织工程研究》2013年第49期8596-8601,共6页Chinese Journal of Tissue Engineering Research

摘　　要：背景:白血病侧群细胞表型的研究对于理解肿瘤细胞的异质性和起源、分子标记和靶向治疗等都有积极意义。目的:鉴定人慢性粒细胞白血病细胞株K562中是否存在侧群细胞,并观察侧群细胞亚群与非侧群细胞亚群中部分白细胞分化抗原的表达差异。方法:采用流式细胞术检测K562细胞株中是否存在侧群细胞;并进一步分析K562侧群细胞和非侧群细胞两亚群间CD34+、CD34+CD38-、CD34+CD38+、HLA-DR+细胞的表达情况。结果与结论:经Hoechst33342染色,流式细胞仪分析结果显示在K562中存在侧群细胞,这部分细胞比例少,为(2.7±0.5)%;统计学分析侧群细胞和非侧群细胞亚群中CD34+、CD34+CD38-细胞表达率差异有显著性意义,而CD34+CD38+细胞表达率和HLA-DR+细胞表达率差异均无显著性意义;侧群细胞和非侧群细胞相比在分化抗原表达上有异质性。BACKGROUND：To study the phenotypes of side population cells in leukemia is important for understanding the heterogeneity and origin of tumor cells, molecular markers and targeted therapy. OBJECTIVE：To identify whether the human chronic myeloid leukemia cellline-K562 contains side population cells or not, and to further observe the differences in expressions of leukocyte differentiation antigens from side population cellsubset and non-side population cells subset. METHODS：Flow cytometry was used to detect whether there were side population cells in the K562 celllines. Then, the expression of CD34^＋, CD34^＋CD38^-, CD34^＋CD38^＋, HLA-DR＋cells in the side population subsets and non-side population subsets. RESULTS AND CONCLUSION：Flow cytometry results showed that the K562 cellline contained side population cells, and the proportion of side population cells was much lower. The side population cells accounted for （2.7±0.5）%of viable cells in K562. The expressions of CD34^＋cells and CD34^＋CD38^-cells in the side population subset were significantly higher than those in the non-side population subsets. The expressions of CD34^＋CD38^＋cells and HLA-DR＋cells in the side population subset and non-side population subset did not have a significant difference. Heterogeneity was found in the differentiation antigen expression between the side population subset and non-side population subset.

关 键 词：干细胞 干细胞培养与分化 白血病干细胞 侧群细胞 非侧群细胞 流式细胞术 K562细胞株 

分 类 号：R394.2[医药卫生—医学遗传学]