人原发胶质母细胞瘤组织中NF-κB、TP53及MGMT甲基化与MGMT蛋白表达的相关性研究  

The Correlations between MGMT Promoter Methylation,NF-κB,TP53 and MGMT Protein Expression in Human Primary Glioblastomas

作　　者：张钧[1] 滕颖[1] 齐雪岭[2] 李岩[1] 于春江[3] 张俊平[1] 

机构地区：[1]首都医科大学第十一临床医学院(北京三博脑科医院)神经肿瘤化疗科,北京100093 [2]首都医科大学第十一临床医学院(北京三博脑科医院)病理科,北京100093 [3]首都医科大学第十一临床医学院(北京三博脑科医院)神经外科,北京100093

出　　处：《中国神经肿瘤杂志》2013年第3期145-150,共6页Chinese Journal of Neuro-Oncology

基　　金：国家临床重点专科建设项目(No.SG2011-03-1-2);神经科学学科群/平台项目

摘　　要：背景与目的:胶质瘤化疗敏感性与一些分子表达相关。本研究检测和分析原发性胶质母细胞瘤(glioblastoma multiforme,GBM)患者肿瘤组织中核因子κB(NF-κB)、突变型P53(TP53)及O6-甲基鸟嘌呤-DNA甲基转移酶(O6-methylguanine-DNA methyltransferase,MGMT)基因启动子区甲基化与MGMT蛋白表达的相关性,以探讨调控MGMT蛋白表达的作用机制。方法:采用甲基化特异性PCR方法 (methylation specific PCR,MSP)检测我院收治的120例原发性GBM患者肿瘤组织标本MGMT基因启动子区甲基化;采用免疫组化方法检测NF-κB、TP53、MGMT蛋白表达情况。采用SPSS17.0软件及Spearman相关系数分析方法进行统计学分析。结果:免疫组化表明NF-κB、TP53表达与MGMT表达呈正相关(r=0.244,r=0.271,P均<0.05),NF-κB与TP53表达亦呈正相关(r=0.329,P<0.05)。MSP结果显示MGMT基因启动子区甲基化率与MGMT蛋白表达强度无相关性。结论:转录因子NF-κB与TP53对原发性GBM肿瘤组织中MGMT蛋白表达可能存在正调控作用,而MGMT基因启动子区甲基化率与MGMT蛋白表达强度无相关性。BACKGROUND ＆ OBJECTIVE： Gene expressions associated with chemosensitivity in human gliomas. This study was to explore the correlations between MGMT promoter methylation , NF-κB, TP53 and MGMT protein expression in the tumor tissue of human primary glioblastoma multiforme, （GBM）. METHODS： The tumor tissues of 120 cases of primary glioblastomas treated in our hospital during June 2008 to August 2011 were collected. The immunohistochemical staining was used for detection of NF-κB, TPJ3 and MGMT protein expressions.The MSP method was applied for the determination of MGMT promoter methylation. RESULTS： The positive correlation was revealed between MGMT protein expression and NF-κB （r = 0.244, P〈 0.05）, also between MGMT protein expression and TP53 （r = 0.271, P〈 0.05）. But no statistical correlation was found between MGMT promoter methylation and MGMT protein expression. CONCLUSION： NF-κB and TP53 may positively regulate the MGMT protein expression in hmnan glioblastomas.

关 键 词：胶质母细胞瘤 NF-ΚB TP53 MGMT基因 

分 类 号：R739.41[医药卫生—肿瘤]