PD-L1 is increased in the spinal cord and infiltrating lymphocytes in experimental allergic encephalomyelitis  

作　　者：Min Li Jiandong Jiang Bing Fu Jiechun Chen Qun Xue Wanli Dong Yanzheng Gu Lingtao Tang Limin Xue Qi Fang Mingyuan Wang Xueguang Zhang 

机构地区：[1]Department of Neurology, Second People's Hospital of Lianyungang City [2]Department of Neurology, First Affiliated Hospital of Soochow University [3]Institute of Clinical Immunology, Soochow University [4]Department of Neurology, Third Hospital of Xingtai City [5]Red-Cross Blood Center of Suzhou City

出　　处：《Neural Regeneration Research》2013年第35期3296-3305,共10页中国神经再生研究（英文版）

基　　金：financially sponsored by the Natural Science Foundation of Jiangsu Province in China,(General Program),No.BK2011267

摘　　要：Experimental allergic encephalomyelitis is a mouse model of human multiple sclerosis with similar pathology and pathogenesis. Thl cells play an important role in the pathogenesis of experimental allergic encephalomyelitis. This study determined the potential effect of programmed cell death 1 ligand 1 in the pathogenesis of experimental allergic encephalomyelitis induced by injecting myelin oligodendrocyte glycoprotein, complete Freund＇s adjuvant and Bordetella pertussis toxin into C57BL/6J mice. Experimental allergic encephalomyelitis mice developed disease and showed in- flammatory changes in the central nervous system by hematoxylin-eosin staining of spinal cord pathological sections, demyelination by Luxol fast-blue staining and clinical manifestations. The expression of programmed cell death 1 ligand 1 in mice was detected by immunohistochemistry, flow cytometry and western blot anatysis. The expression of programmed cell death 1 ligand 1 in the spinal cord and splenocytes of mice was significantly increased compared with normal mice. Our findings suggest the involvement of programmed cell death 1 ligand 1 in the pathogenesis of ex- perimental allergic encephalomyelitis and suggest this should be studied in multiple sclerosis.Experimental allergic encephalomyelitis is a mouse model of human multiple sclerosis with similar pathology and pathogenesis. Thl cells play an important role in the pathogenesis of experimental allergic encephalomyelitis. This study determined the potential effect of programmed cell death 1 ligand 1 in the pathogenesis of experimental allergic encephalomyelitis induced by injecting myelin oligodendrocyte glycoprotein, complete Freund＇s adjuvant and Bordetella pertussis toxin into C57BL/6J mice. Experimental allergic encephalomyelitis mice developed disease and showed in- flammatory changes in the central nervous system by hematoxylin-eosin staining of spinal cord pathological sections, demyelination by Luxol fast-blue staining and clinical manifestations. The expression of programmed cell death 1 ligand 1 in mice was detected by immunohistochemistry, flow cytometry and western blot anatysis. The expression of programmed cell death 1 ligand 1 in the spinal cord and splenocytes of mice was significantly increased compared with normal mice. Our findings suggest the involvement of programmed cell death 1 ligand 1 in the pathogenesis of ex- perimental allergic encephalomyelitis and suggest this should be studied in multiple sclerosis.

关 键 词：neural regeneration experimental allergic encephalomyelitis multiple sclerosisautoimmune disease costimulatory signal costimulatory molecule programmed ce1 B7-CD28 superfamily grants-supported paper neuroregenerationanimal models II death 1 ligand 

分 类 号：R744.3[医药卫生—神经病学与精神病学]