抗氧化剂与骨髓细胞移植对改善糖尿病小鼠缺血下肢血流效果的比较  

Comparision of blood flow recovery of the ischemic limbs between antioxidant therapy and bone marrow cells implantation for diabetes mouse

作　　者：秦淑兰[1] 黄承洲 朱艳[1] 蔡霞[3] 赖晓阳[3] 

机构地区：[1]南昌大学第三附属医院,南昌330008 [2]江西丰城矿务局总医院,丰城331141 [3]南昌大学第二附属医院,南昌330006

出　　处：《江西医药》2013年第11期970-972,共3页Jiangxi Medical Journal

摘　　要：目的比较抗氧化剂和骨髓细胞移植对改善糖尿病小鼠缺血下肢血流的效果。方法将18只糖尿病小鼠均分成3组,抗氧化剂组给予N-acetyl-L-cysteine(NAC),200mg/(kg·d)腹腔内投药治疗4周后,建立下肢缺血模型,将PBS注入缺血侧下肢的肌肉内。骨髓细胞移植组和对照组给予生理盐水腹腔内投药治疗4周后,建立下肢缺血模型,分别将骨髓细胞或PBS注入缺血侧下肢的肌肉内。在缺血下肢建立后的第1d和第7d,使用彩色多普勒测量小鼠下肢股动脉及其分支血流变化。结果与正常对照组比较,抗氧化剂组和骨髓细胞移植组下肢血流均明显改善(P<0.05);但是,与抗氧化剂组比较,骨髓细胞移植组下肢血流改善更显著(P<0.05)。结论骨髓细胞移植比抗氧化剂治疗糖尿病合并缺血性疾病更有效。Objective To compare the therapeutic effect on ischemic limbs between antioxidant therapy and bone marrow cells implantation for diabetes mouse. Methods 18 diabetic mice were divided into 3 groups. In the antioxidant group, after injecting subcutaneous with N-acetyl-L-cysteine（NAC） 200mg/（kg·d） for 4 weeks,the ischemic limb muscle was injected with PBS. In the BMCI and control groups,after the vehicle treatment for 4 weeks,the ischemic limb muscle was injected with 6×10^6 bone marrow ceils in the BMCI group,or with PBS in the control group. Blood flow was measured by using the laser doppler perfusion image system （PeriScan System,Perimed, Stockhohn,Sweden） at 1 and 7 days after the induction of limb ischemia. Results Compared with the control group, the limb blood flow （LBF） was significantly higher both in the antioxidant and BMCI groups （P〈0.05）. However,the LBF was significantly higher in the BMCI group than in the antioxidant group （P〈0.05）. Conclusion BMCI is more effective method than antioxidant therapy of inducing therapeutic angiogenesis for ischemic diseases with diabetes.

关 键 词：抗氧化剂 骨髓细胞移植 缺血性疾病 糖尿病 

分 类 号：R-332[医药卫生]