过表达HMGA2对人垂体生长激素腺瘤细胞增殖及周期的影响  

The effect of overexpression HMGA2 on proliferation and cell cycle in human growth hormone-secreting pituitary tumor cells

作　　者：范月超[1] 颜丙超[1] 陈洪福[1] 纪建永[1] 张慧[1] 梅鹏金[1] 

机构地区：[1]徐州医学院附属医院颅底肿瘤外科,江苏徐州221006

出　　处：《重庆医学》2013年第36期4371-4373,共3页Chongqing medicine

基　　金：江苏省卫生厅科研基金资助项目(H201019)

摘　　要：目的探讨过表达高迁移率族蛋白A2(HMGA2)对人垂体生长激素腺瘤细胞增殖及周期的影响。方法 HMGA2过表达质粒转染原代培养人垂体生长激素腺瘤细胞(转染组)。蛋白免疫印迹法(Western blot)、细胞增殖实验(cell counting kit-8,CCK-8)、流式细胞仪术分别检测HMGA2过表达质粒转染垂体生长激素腺瘤细胞后对HMGA2蛋白表达、细胞增殖、细胞周期的影响。结果 24h后转染组垂体生长激素腺瘤细胞HMGA2蛋白表达水平高于空载体组(转染空质粒的细胞)(P<0.05);较空载体组在24、48、72、96h4个时间点OD值较大,差异均有统计学意义(P<0.05);转染组细胞增殖数量增加,与空载体组相比转染组细胞G1期所占比例减少,S期所占比例增加,差异有统计学意义(P<0.05)。结论过表达HMGA2促进垂体腺瘤细胞增殖,促进细胞G1期进展。Objective To explore the effects of over-expression high-mobility-group A2（HMGA2） on proliferation and cell cycle in primary cultured human growth hormone-secreting pituitary tumor cells .Methods The protein expression of HMGA2 in growth hormone-secreting pituitary tumor cells transfected by HMGA2 overexpression plasmid （transfection group） were detected by Western blot .The effects of the HMGA2 overexpression on cell proliferation ,cell cycle were measured by （cell counting kit-8 , CCK-8） assay and flow cytometry respectively .Results HMGA2 protein of transfection group was higher than the blank load transfection group（tranfectied with pcDNA3 .1 plasmid） after 24 h （P〈0 .05） .To compare with the blank load transfection group , cells overexpression of HMGA2 could drastically increase the ability of proliferation at 24 ,48 ,72 ,96 h and increase the S phase rati-o of cell cycle and decrease the G1 phase ratio of cell cycle ,the difference was statistically significant （P〈0 .05） .Conclusion Over-expression of HMGA2 increases pituitary tumor cells proliferation ability and promote cells G 1 phase progress .

关 键 词：高迁移率族蛋白类 增量调节 垂体肿瘤 增殖 细胞周期 

分 类 号：R736.4[医药卫生—肿瘤]