胃泌素对胃癌细胞MMP-2和E-Cadherin表达模式的影响  

The study on the expression pattern of MMP-2 and E-Cadherin in the effects of gastrin on gastric cancer cells

作　　者：张宇晴[1] 徐优慧[2] 黎文婷[3] 邹学森[1] 罗达亚[4] 陈文学[1] 黄秀珍[1] 

机构地区：[1]江西省肿瘤医院检验科,南昌330029 [2]江西中医药大学病理教研室,南昌330004 [3]江西省儿童医院,南昌330006 [4]南昌大学医学院生化与分子生物学教研室,南昌330006

出　　处：《重庆医学》2013年第36期4374-4376,4379,共4页Chongqing medicine

基　　金：江西省卫生厅科技计划课题(20093095)

摘　　要：目的探讨胃泌素对胃癌细胞基质金属蛋白酶-2(MMP-2)和E-钙粘蛋白(E-Cadherin)表达模式的影响,及其在胃癌转移及侵袭中的作用。方法以亚硫酸氢盐修饰后的基因组DNA为模板,运用实时定量甲基化特异性PCR(RQ-MSP)法检测各组基因甲基化状态,通过比较Ct(2-△t)进行甲基化相对量分析。结果胃癌细胞在胃泌素干预前、后MMP-2和E-Cadherin基因均处于半甲基化状态;胃泌素可显著降低MMP-2基因甲基化水平,差异有统计学意义(P<0.05),其受体拮抗剂丙谷酰胺可一定程度抑制其效应(P>0.05);胃泌素可明显提高E-Cadherin甲基化水平,而其受体拮抗剂丙谷酰胺可显著降低甲基化水平,差异有统计学意义(P<0.05)。结论胃泌素可以通过改变MMP-2和E-Cadherin表达模式,从而在胃癌侵袭转移的生物学行为中发挥一定作用。Objective To approach on the expression pattern of MMP-2 and E-Cadherin in the effects of gastrin on gastric canc-er cells ,so as to investigate their role in the metastasis and invasion of gastric cancer .Methods Genomic DNA modified by sodium bisulfite was used as a template .RQ-MSP detect the methylation status of the gene promoter .The relative amount of Methylation status was performed with comparative threshold cycle （2- △ t ） method .Results MMP-2 and E-Cadherin genes in gastric cancer cells were at hemimethylated status in gastrin before and after the intervention .Gastrin can reduce the methylation levels of MMP-2 gene（P〈0 .05） ,but the could be effected by proglumide receptor antagonist to some certain extent （P〉0 .05） .Gastrin can pro-mote the methylation levels of MMP-2 gene ,but proglumide decrease that effect （P〈0 .05） .Conclusion Gastrin plays a certain role in the biological behavior of gastric cancer impact by changeing on the expression pattern of MMP-2 and E-Cadherin in the effects of gastrin on gastric cancer cells .

关 键 词：胃泌素类 丙谷酰胺 甲基化 基质金属蛋白酶2 钙黏着蛋白类 

分 类 号：R735.2[医药卫生—肿瘤]