上调C/EBPα对PAR1激活的HSC-T6细胞的影响  

The effect of over-expression of C/EBPαon HSC-T6 cell actived by PAR1

作　　者：刘迎春[1] 顾小红[1] 张云东[2] 段绪伟[1] 

机构地区：[1]第三军医大学大坪医院野战外科研究所高干科,重庆400042 [2]第三军医大学大坪医院野战外科研究所神经外科,重庆400042

出　　处：《重庆医学》2013年第36期4424-4426,共3页Chongqing medicine

基　　金：重庆市自然科学基金资助项目(CSTC:2010BB5165);中国人民解放军第三军医大学中青年基金(XG200543)

摘　　要：目的探讨上调CCAAT增强子结合蛋白α(C/EBPα)对蛋白酶活化受体1(PAR1)激活的肝星状细胞(HSC)活化、增殖和分泌胶原蛋白的影响。方法瞬时转染法转染经PAR1激活的HSC-T6,根据转染质粒的加PAR1活性肽的情况将细胞分为正常组(未做任何处理)、SFLLR组、SFLLR+C/EBPα质粒组、SFLLR+空载体组。蛋白质印迹法(Western-blot)检测转染后24、48hHSC内α平滑肌动蛋白(α-SMA)及Ⅰ型前胶原的表达,MTT检测细胞增殖度。结果 SFLLR+C/EBPα质粒组细胞内α-SMA及Ⅰ型前胶原蛋白的表达较其余各组明显减少,细胞增殖度也明显下降,均以转染后48h明显改变(P<0.01)。结论上调C/EBPα的表达在一定程度上可抑制PAR1对HSC-T6的活化、增殖和胶原蛋白的合成。Objective To explore the over-expression of the CCAAT enhancer binding proteinα（C/EBPα）in hepatic stellate cells （HSC-T6） which activated by protease-activated receptor 1（PAR1） ,to explore its possible relationship with cell proliferation ,cell activation and collagen secretion of HSC-T6 cell .Methods HSC-T6 cells were divided into 4 groups ,control group ,SFLLR group;vehicle plasmid＋SFLLR group ,C/EBPαplasmid＋SFLLR group .The eukaryotic vector harboring the full length of C/EBPαcD-NA was transfected into PAR1 activated HSC-T6 cell ,then Western blot was applied to detect the expression of α-smooth muscle actin（α-SMA） and collagen I and evaluate the effect of transfection on proliferation of HSC-T6 by MTT .Results The expression of α-SMA and collagen I in C/EBPαplasmid＋SFLLR group were dramatically decreased compared with other 3 groups ,as well as proliferation after 48 h（P〈0 .05） .Conclusion Over-expression of C/EBPαgene by transfection had inhibitory influence on prolif-eration ,activation and collagen secretion of HSC-T6 which were activated by PAR1 .

关 键 词：CCAAT增强子结合蛋白α 受体 RAP-1 肝星状细胞 

分 类 号：R363[医药卫生—病理学]