Cloning and Functional Analysis of A NAC-Like Gene Csnac in Tea Plant(Camellia sinensis)  

作　　者：Lei Tang Zheng-zhong Jiang Jie-Ru Zhang Chao-Ling Wei 

机构地区：[1]Key Laboratory of Tea Biochemistry and Biotechnology,Ministry of Education and Ministry of Agriculture,Anhui Agricultural University

出　　处：《茶叶》2013年第4期279-286,共8页Journal of Tea

基　　金：supported by the National Natural Science Foundation of China project (306713312)

摘　　要：The plant-specific NAC(NAM,ATAF1 /2,CUC2) transcription factors play important roles in stess responses and devlopment in plants,but little was known about NACs in tea plants(Camellia sinensis).In this study,a fulllength cDNA sequence(CsNAC) was isolated by suppressive subtractive hybridization(SSH) in Ectropic oblique feeding-induced tea plants.Gene sequence analysis showed that the putative protein of CsNAC contained a conserved NAC domain,which is the typical characteristic of NAC transcription factors.qRT-PCR analysis revealed that CsNAC was suppressed by Ectropis oblique feeding and mechanical damage.Treatment of defence-related homones methyl jasmonate(MeJA) decreased the transcript abudance of CsNAC and salicylic acid(SA) could induce the expression of CsNAC.The transcript levels of CsNAC was down-regulated after applying abscisic acid(ABA) and the expression patterns of CsNAC were different by drought or cold stress.Subcellular localization analysis revealed that CsNAC protein was localized in the nucleus.These results suggested that CsNAC may play a role in defense against insects and adaptation to abiotic stresses.The plant-specific NAC （NAM,ATAF1/2,CUC2） transcription factors play important roles in stess responses and devlopment in plants,but little was known about NACs in tea plants （Camellia sinensis）.In this study,a fulllength cDNA sequence （CsNAC） was isolated by suppressive subtractive hybridization （SSH） in Ectropic oblique feeding-induced tea plants.Gene sequence analysis showed that the putative protein of CsNAC contained a conserved NAC domain,which is the typical characteristic of NAC transcription factors.qRT-PCR analysis revealed that CsNAC was suppressed by Ectropis oblique feeding and mechanical damage.Treatment of defence-related homones methyl jasmonate （MeJA） decreased the transcript abudance of CsNAC and salicylic acid （SA） could induce the expression of CsNAC.The transcript levels of CsNAC was down-regulated after applying abscisic acid （ABA） and the expression patterns of CsNAC were different by drought or cold stress.Subcellular localization analysis revealed that CsNAC protein was localized in the nucleus.These results suggested that CsNAC may play a role in defense against insects and adaptation to abiotic stresses.

关 键 词：茶树 E基因 RT-PCR分析 克隆 抑制消减杂交 CDNA序列 基因序列分析 转录因子 

分 类 号：S571.1[农业科学—茶叶生产加工]