SCCAg、CK19mRNA与早期宫颈癌前哨淋巴结微转移的相关性研究  

Study of sentinel lymph node micrometastasis by combining detection SCCAg and CK19mRNA in early stage cervical cancer

作　　者：马永静[1] 熊英[1] 卜祥静 刘瑞[1] 

机构地区：[1]宁夏医科大学总医院妇科,宁夏银川750004 [2]宁夏医科大学,宁夏银川750004

出　　处：《宁夏医学杂志》2013年第11期1038-1040,共3页Ningxia Medical Journal

基　　金：宁夏医科大学面上项目(XM201034)

摘　　要：目的探讨鳞状细胞癌抗原(SCCAg)联合细胞角蛋白(CKs)19mRNA在预测IA2-IB2期宫颈癌前哨淋巴结微转移中的价值及相关性。方法 IA2-IB2期宫颈癌患者为研究组,CIN患者及健康妇女为对照组。用ELISA法检测宫颈癌患者术前及对照组血清中SCCAg含量,荧光定量聚合酶链反应(FQ-PCR)检测宫颈癌患者前哨淋巴结CK19mRNA的表达,分析两项指标的临床价值及两者间的关系。结果宫颈癌组血清SCCAg较CIN组及健康体检组明显增高;荧光定量PCR检测CK19mRNA检出前哨淋巴结微转移的阳性率(32.6%)比常规病理检查(12.8%)高;血清SCCAg与CK19mRNA表达呈正相关。结论检测CK19mRNA能显著提高IA2-IB2期宫颈癌前哨淋微转移的检出率;联合检测IA2-IB2期宫颈癌患者血清SCCAg及前哨淋巴结CK19mRNA可能成为预测早期宫颈癌前哨淋巴结发生微转移的一种有价值的方法。Objective To investigate the clinical value and correlation of combining SCCAg with CK19mRNA in sentinel lymph node micrometastasis of early stage cervical cancer. Methods Serum SCCAg was detected in patients with stage IA2 - IB2 squamous cell cervical carcinoma,patients with CIN and healthy women, respectively. CK19mRNA was detected by FQ -PCR in sentinel lymph nodes of patients with cervical cancer, and relationship between the two biomarkers was studied, too. Results Serum SCCAg level of pa- tients with cervical cancer was higher than that in control group ; detection rate of sentinel lymph node micrometastasis by FQ - PCR （32.6%） was significantly higher than histopathology （12. 8% ）;there was direct correlation between SCCAg and CK19. Conclusion FQ- PCR is better than histopathology in detection of CK19mRNA in sentinel lymph node micrometastasis of cervical cancer; combination of these two markers is likely to become a valuable method to predict sentinel lymph node micrometastasis of early stage cervical cancer.

关 键 词：鳞状细胞癌抗原 细胞角蛋白19mRNA IA2-IB2期宫颈癌 前哨淋巴结 微转移 

分 类 号：R737.33[医药卫生—肿瘤]