结直肠癌患者血清中转甲状腺素蛋白的表达与肿瘤转移的相关性  

Expression of Serum Transthyretin and Its Relevance to Metastasis in Colorectal Cancer

作　　者：胡慧慧[1] 孟倩[1] 张曼[1] 

机构地区：[1]首都医科大学附属北京世纪坛医院临床检验中心,北京100038

出　　处：《标记免疫分析与临床》2013年第6期378-380,共3页Labeled Immunoassays and Clinical Medicine

基　　金：北京自然科学基金:膀胱癌尿液蛋白生物标记物的研究(7122086);国家科技支撑计划(2013BAI12B01-4)

摘　　要：目的对发生远处转移结直肠癌患者血清中转甲状腺素蛋白(transthyretin,TTR)的表达进行研究,旨在探讨TTR的表达与结直肠癌转移的关系,进一步发现TTR与结直肠肿瘤转移之间的相关性。方法收集23例结直肠癌患者血清,标本于-80℃保存。根据是否发生远处转移进行分类,未发生远处转移样本12例,远处转移样本11例。采用蛋白免疫印迹的方法对TTR的表达进行半定量分析。结果利用Image J软件计算各组光密度比值,显示在未发生远处转移的结直肠癌患者血清中,TTR的表达量高于发生远处转移的患者,TTR在两组结直肠癌患者血清中表达差异有统计学意义(P=0.017)。结论 TTR在结直肠癌患者血清中的表达水平与肿瘤是否发生转移存在相关性,因此,今后对于评估结直肠癌浸润与转移的水平,TTR可以发挥肿瘤标志物的作用。Objective To investigate the expression of serum transthyretin （TFR） in colorectal cancer patients with distant metastasis （live＆ stomach metastasis）, so as to find different expression of transthyretin between two groups, to explore expression of transthyretin and its relevance to metastasis of colorectal cancer. Methods The serum of colorectal cancer patients including occur metastasis 12 cases and patients with metastasis 11 cases were collected and stored in -80℃ until use. Expression of serum TTR was semi-quantitative identified by using western blotting method. Software Image J analyzed the strip light density. Results The expression of ser- um TTR in coloreetal cancer patients with metastasis was lower than patients without metastasis （ P = 0.017 ）. Conclusion The correlation between serum TTR and metastasis of colorectal cancer could provide clinical aid to identify the tumor aggression and migration, therefore, the TTR could be used as a tumor maker in evaluation of invasion and metastasis of colorectal cancer.

关 键 词：转甲状腺素蛋白 转移 结直肠癌 蛋白免疫印迹 

分 类 号：R735.3[医药卫生—肿瘤]