被动吸烟所致小鼠肺癌中Angiopoietin-3基因过表达及其意义的初步探讨  

Angiopoietin-3 overexpression in tobacco smoke-induced mouse lung tumors

作　　者：张锁连[1] 宋腾腾 梁琨[1] 陈照立[3] 

机构地区：[1]烟台经济技术开发区医院病理科,264006 [2]山东栖霞人民医院病理科,265399 [3]军事医学科学院卫生学环境医学研究所,天津300050

出　　处：《武警医学》2013年第12期1056-1060,共5页Medical Journal of the Chinese People's Armed Police Force

摘　　要：目的初步寻找新的与肺癌相关的基因,为全面揭开肺癌发病机制、进一步诊断和治疗肺癌提供依据。方法 (1)通过基因表达芯片筛选被动吸烟致小鼠肺癌中异常表达的基因;(2)荧光实时定量PCR验证部分基因芯片结果;(3)应用western blotting分析方法检测小鼠肺组织中Angiopoietin-3(Ang-3)基因的蛋白表达量变化。结果实时荧光定量PCR结果显示Ang-3基因的mRNA相对表达量在肺癌组(30.6544±15.2245)明显高于在正常对照组(1.0469±0.3441,P<0.05)。Western blotting结果进一步表明吸烟导致Ang-3基因蛋白高表达(1.2724±0.1493),尤其是在肺癌组织中(1.5901±0.1189)明显高于正常肺组织中(0.9054±0.0523),差异有统计学意义(P<0.05)。结论 Ang-3基因在被动吸烟所致小鼠肺癌的发生、发展中发挥一定的作用。Objective To provide evidences fbr searching initially new correlated genes of lung cancer, and to reveal lung ear- cinogenesis, development and prognosis. Methods 1.To screen the genes of anomalous expression in mouse lung cancer induced by tobacco smnke through gene expression ehiI） of mouse genome; 2. To identify the partial results of gene chip by fluorescence - based re- al - time PCR ; 3. To detecting the protein expression changes of Ang- 3 in mouse lung cancer by western Blotting essay. Results Real- time PCR showed that Ang-3 gene presented higher expression in the tumor group （30. 6544 ±15. 2245 ）than in the control normal group（ 1. 0469 ±0. 3441 ）, P 〈 0.05. Western blotling assay further indicated that smoking couht upregulate the expression of Aug - 3 （ 1. 2724 ± 0. 1493） ; moreover, Ang- 3 was overexpressed in lung cancer tissue （ 1. 5901 ± 0. 1189 ） than in normal tisuue （0. 9054 ±0. 0523 ） , P 〈 0. 05. Conclusions Ang- 3 gene plays an important role in mouse lung cancer induced by tobacco smoke.

关 键 词：肺癌 基因 实时荧光定量PCR WESTERN BLOTTING Angiopoietin-3 

分 类 号：R734.2[医药卫生—肿瘤]