LED光照对拟南芥叶绿素含量和根生长发育的影响  

The Effects of LED Light on the Chlorophyll Content and Root Growth of Arabidopsis

作　　者：刘颖[1] 林娟[2] 周小丽[1] 刘木清[1] 

机构地区：[1]复旦大学电光源研究所,先进照明技术教育部工程研究中心,上海200433 [2]复旦大学遗传工程国家重点实验室,生命科学学院,上海200433

出　　处：《复旦学报（自然科学版）》2013年第6期762-767,共6页Journal of Fudan University：Natural Science

基　　金：国家高技术研究发展计划(863计划)资助项目(2011AA03A114)

摘　　要：以模式植物拟南芥为研究材料,根据植物叶片中叶绿素a和b对可见光的吸收曲线,选取主波长为445nm和638nm单色红蓝LED光源为培养条件,观察了拟南芥在荧光灯或LED灯下的生长特性.结果显示:当光量子通量密度在64μmol·m-2·s-1附近时,荧光灯和LED灯下的叶绿素a+b平均含量分别为1.45mg·g-1和1.52mg·g-1,且叶绿素a、b的含量随红蓝光比例的升高有降低趋势;LED光照下的拟南芥主根长度比在荧光灯条件下约长20mm,且其侧根数量多于在荧光灯下的侧根数量.在LED光照环境下,当光量子通量密度从14μmol·m-2·s-1到375μmol·m-2·s-1变化时,拟南芥根系长度在200μmol·m-2·s-1时达到最高,并随着光量子通量密度升高而逐渐降低,而侧根的数量则随着光量子通量密度的增高而升高.Based on the absorption spectrum of the chlorophyll a and b, and the advantage of the new generation light source Light-emitting diodes （LED）, an experiment was designed to study the chlorophyll content and root growth characteristics of Arabidopsis. Different red to blue light ratio （R/B） and different Photosynthetic Photon Flux Density （PPFD） light environments were established by using blue LED with dominated wavelength of 454 nm and red LED with dominated wavelength of 638 nm. A Fluorescent light environment was also established for comparison. The experiment results show that under the PPFD of about 64 μmol·m^-2·s^-1 condition, the average chlorophyll content Of Arabidopsis under LED light and that under fluorescent lamp light are 1.52 mg-g 1 and 1.45 mg.g-1 respectively; Chlorophyll a, b and a＋b contents become lower as the increase of the R/B ratio. Also in 64 μmol·m^-2·s^-1 PPFD LED light environment, the average main root length （RL） of Arabidopsis under the environment of LED light is 20 mm longer than that under fluorescent lamps. The number of lateral root is higher in LED light environment too. Under LED lighting environment, when the total PPFD changes from 14 μmol·m^-2·s^-1 to 375 μmol·m^-2·s^-1 , the main root length becomes longer, and then decrease when PPFD is higher than 200 μmol·m^-2·s^-1.

关 键 词：LED根长 荧光灯 光谱分析 叶绿素 

分 类 号：S123[农业科学—农业基础科学]