人血管生成素-1真核表达载体的构建与表达  

Construction and expression of human angiopoietin-1 eukaryotic vector

作　　者：吕永强[1] 童贻刚[2] 

机构地区：[1]解放军第210医院检验科,辽宁大连116021 [2]解放军军事医学科学院微生物流行病研究所,北京100071

出　　处：《临床军医杂志》2013年第12期1234-1236,共3页Clinical Journal of Medical Officers

摘　　要：目的构建人血管生成素-1的真核表达载体,真核表达人血管生成素-1蛋白并进行纯化。方法通过聚合酶链反应(PCR)将人血管生成素-1基因片段插入B7载体中构建真核表达载体,通过转染中国仓鼠卵巢(CHO)细胞进行真核表达。结果成功构建了B7Ang-1真核表达载体,在CHO细胞中进行了稳定表达,获得了纯化的人血管生成素-1蛋白。结论真核系统表达的人血管生成素-1蛋白具有良好的抗原性,为进一步的生物活性研究和临床应用奠定了基础。Objective To construct the eukaryotic expression vector of human angiopoietin-1, and express and purify the human angiopoietin-1 protein. Methods The fragment of human angiopoietin-1 gene was inserted into B7 vector by polymerase chain reaction （PCR） and then transfected into Chinese hamster ovary （CHO） cells for eukaryotic expression. Results The B7Ang- 1 eukaryotic vector was constructed and stably expressed in CHO cell. The purified human angiopoietin-1 protein was obtained. Conclusion Human angiopoietin-1 protein is expressed in the eukaryotic system with good antigenicity. This study lays a good foundation for further study on the biological activity and clinical application of human angiopoietin-1.

关 键 词：人血管生成素-1 聚合酶链反应 真核表达 

分 类 号：R730.43[医药卫生—肿瘤]