Lipase-catalyzed Synthesis of Caffeic Acid Phenethyl Ester in Ionic Liquids： Effect of Specific Ions and Reaction Parameters  

作　　者：王俊 李晶 张磊霞 顾双双 吴福安 

机构地区：[1]School of Biology and Chemical Engineering, Jiangsu University of Science and Technology, Zhenjiang 212018, China [2]Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018, China

出　　处：《Chinese Journal of Chemical Engineering》2013年第12期1376-1385,共10页中国化学工程学报（英文版）

基　　金：Supported by the Natural Science Foundation of Jiangsu Province（BK2009213）; China Postdoctoral Science Foundation funded Project（2012M510124）; Qing Lan Project of Jiangsu Province,National Natural Science Foundation of China（21206061）; Research Project of Jiangsu University of Science and Technology（35211002）; Modern Agro-industry Technology Research System of China（CARS-22）

摘　　要：Caffeic acid phenethyl ester(CAPE)is a rare,naturally occurring phenolic food additive.This work systematically reported fundamental data on conversion of caffeic acid(CA),yield of CAPE,and reactive selectivity during the lipase-catalyzed esterification process of CA and phenylethanol(PE)in ionic liquids(ILs).Sixteen ILs were selected as the reaction media,and the relative lipase-catalyzed synthesis properties of CAPE were measured in an effort to enhance the yield of CAPE with high selectivity.The results indicated that ILs containing weakly coordinating anions and cations with adequate alkyl chain length improved the synthesis of CAPE.[Emim][Tf2N]was selected as the optimal reaction media.The optimal parameters were as follows by response surface methodology(RSM):reaction temperature,84.0°C;mass ratio of Novozym 435 to CA,14︰1;and molar ratio of PE to CA,16︰1.The highest reactive selectivity of CAPE catalyzed by Novozym 435 in[Emim][Tf2N]reached 64.55%(CA conversion 98.76%and CAPE yield 63.75%,respectively).Thus,lipase-catalyzed esterification in ILs is a promising method suitable for CAPE production.Caffeic acid phenethyl ester（CAPE） is a rare, naturally occurring phenolic food additive. This work systematically reported fundamental data on conversion of caffeic acid（CA）, yield of CAPE, and reactive selectivity during the lipase-catalyzed esterification process of CA and phenylethanol（PE） in ionic liquids（ILs）. Sixteen ILs were selected as the reaction media, and the relative lipase-catalyzed synthesis properties of CAPE were measured in an effort to enhance the yield of CAPE with high selectivity. The results indicated that ILs containing weakly coordinating anions and cations with adequate alkyl chain length improved the synthesis of CAPE. [Emim][Tf2 N] was selected as the optimal reaction media. The optimal parameters were as follows by response surface methodology（RSM）： reaction temperature, 84.0 °C; mass ratio of Novozym 435 to CA, 14︰1; and molar ratio of PE to CA, 16 ︰ 1. The highest reactive selectivity of CAPE catalyzed by Novozym 435 in [Emim][Tf2 N] reached 64.55%（CA conversion 98.76% and CAPE yield 63.75%, respectively）. Thus, lipase-catalyzed esterification in ILs is a promising method suitable for CAPE production.

关 键 词：BIOCATALYSIS caffeic acid phenethyl ester ESTERIFICATION ionic liquid LIPASE response surface methodology 

分 类 号：TQ645.98[化学工程—精细化工] S896.6[农业科学—特种经济动物饲养]