结肠癌组织中蛋白酶活化受体-1的表达及意义  

Clinical significance of PAR-1 mRNA expression in patients with colorectal carcinoma

作　　者：汪宏梅[1] 

机构地区：[1]江苏省泰兴市人民医院检验科,江苏泰兴225400

出　　处：《国际检验医学杂志》2013年第24期3320-3321,3323,共3页International Journal of Laboratory Medicine

摘　　要：目的 探讨结肠癌组织中蛋白酶活化受体-1（PAR-1）mRNA的表达情况及其临床意义.方法 实时荧光定量PCR（RT-PCR）检测33例结肠癌患者癌组织及其相应癌旁组织中PAR-1 mRNA的表达情况,分析PAR-1 mRNA与结肠癌分化程度、病理分期以及转移等指标的相关性.结果 结肠癌组织中PAR-1 mRNA的表达明显增高,比邻近癌旁组织平均高4.99倍;二者ΔCt比较,t=9.15,P〈0.01.同时低分化的结肠癌患者组PAR-1 mRNA表达量明显高于中、高分化患者组,P〈0.01;有转移患者组的PAR-1 mRNA表达量明显高于无转移患者组,P〈0.01;而在结肠癌临床Ⅰ~Ⅱ期与Ⅲ~Ⅳ期患者组之间,PAR-1 mRNA表达量差异无统计学意义,P〉0.05.结论 PAR-1 mRNA在结肠癌中高表达,且与结肠癌的分化及是否转移有关.Objective To explore the expression of protease-activated receptor-1（PAR-1） mRNA in colorectal carcinoma（CRC） and its clinical significance. Methods Tissue samples,including 33 cancerous and adjacent non-cancerous tissues were obtained from CRC patients who underwent surgical resection. The expression of PAR-1 mRNA was analyzed by real-time PCR. The relationship between PAR-1 mRNA and clinicopathological features was also evaluated. Results The expression of PAR-1 mRNA in CRC was 4.99 times higher than that in the corresponding adjacent non-cancerous tissues（P〈0. 05）. Moreover, the expression of PAR-1 mRNA in poorly differentiated CRC patients was significantly higher than that of moderately and well-differentiated group（P〈0.01）. The expression of PAR-1 mRNA in metastasis group was obviously higher than that in without metastasis group （P〈0.01）. However,no significantly difference was observed between the groups in the clinical stage Ⅰ - Ⅱ and Ⅲ- Ⅳ （P〉0.05）. Conclusion PAR-1 mRNA has a high expression in coloreetal tissues,and has a relationship with the degree of differentiation and metastasis.

关 键 词：蛋白酶活化受体-1 结肠癌 实时荧光定量聚合酶链反应 凝血酶 

分 类 号：R735.35[医药卫生—肿瘤]