犊牛卵泡液多肽信息分析  

Polypeptide information in follicular fluids of calves

作　　者：赵海峰[1] 柯浩[1] 张飒[1] 陈东峰[1] 陈集成[1] 陈宝剑[1] 沈创[2] 韦英将[2] 蒋钦杨[1] 陆凤花[1] 韦英明[1] 

机构地区：[1]广西大学动物繁殖研究所/亚热带农业生物资源保护与利用国家重点实验室,南宁530005 [2]广西大学南方良种牛繁育中心,南宁530005

出　　处：《南方农业学报》2013年第11期1909-1913,共5页Journal of Southern Agriculture

基　　金：广西自然科学基金项目(2013GXNSFAA019095)

摘　　要：【目的】了解成年牛和犊牛卵泡液中多肽的信息表达差异,为完善犊牛卵泡液基础研究及犊牛卵母细胞体外培养体系奠定基础。【方法】采用基质辅助激光解吸附电离飞行时间质谱技术(MALDI-TOF-MS)对超排成年牛和超排犊牛卵泡液中的肽段进行分析,根据多肽的信号峰信息,通过MASCOT数据库进行蛋白检索。【结果】从成功构建的成年牛与犊牛卵泡液质谱多肽信号峰模型图谱可知,超排犊牛卵泡液多肽表达图谱中有60～80个信号峰,而超排成年牛的有30～40个信号峰;其中成年牛卵泡液有两个特有信号峰,质荷比为1218.58和1509.77 m/z,犊牛有1个特有信号峰,质荷比为953.54 m/z。利用MASCOT数据库对获得的卵泡液多肽信息进行蛋白检索,发现成年牛卵泡液中有18种蛋白,犊牛卵泡液中有11种蛋白。【结论】犊牛卵泡液中蛋白多肽信息峰数量多于成年牛,但卵泡液中总蛋白数量少于成年牛,这种差异可能是导致犊牛卵母细胞发育能力低于成年牛的重要原因之一。[Objective]Different expression profiles of polypeptides in follicle fluid from superovulated cattle and calves were studied in order to provide references for further research on follicle fluid of calves and in vitro oocyte culture system of calves.[Method]The matrix-assisted laser desorption ionization mass spectrometry （MALDI-TOF MS）was used to analyze the peptide fragments in follicular fluid of superovulated cattle and calves.Based on signal peak information of polypeptide,protein search was conducted in MASCOT database.[Result]The models of polypeptide expression profiles of the two groups were successfully established,respectively.There were 30-40 peak signals in cattle group,and 60-80peak signals in calves.By comparing the peak lists from the two different samples,it was found that 2 specific peaks of 1218.58 and 1509.77 m/z appeared in cattle group,similarly,and 1 specific peak of 953.54 m/z in calf group.At last,MASCOT library was used to search the information of polypeptides,and then some proteins related to these polypeptides information were gained.The results showed thai 18 kinds of proteins existed in cattle group,and 11 kinds of proteins in calves.[Conclusion]The results showed that the polypeptide information peak quantity of calf follicular fluid protein was higher than that of adult cattle,while the total number of proteins in follicle fluid from the calves was less than that of the adult cattle group,which could be one of reasons why the developmental competence of oocytes from calves was poorer than that of adult cattle.

关 键 词：犊牛 卵泡液 超数排卵 多肽 基质辅助激光解吸附电离飞行时间质谱技术(MALDI-TOF-MS) 

分 类 号：S814.1[农业科学—畜牧学]