牡丹皮中3种活性成分提取方法优化及含量测定  

Extraction optimization and content determination of three effective ingredients in Moutan Coretex

作　　者：赵亮[1] 吴豪[2] 张海[1] 吕磊[1] 朱臻宇[2] 张国庆[1] 柴逸峰[2] 

机构地区：[1]第二军医大学附属东方肝胆外科医院药材科,上海200438 [2]笫二军医大学药学院,上海200433

出　　处：《药学实践杂志》2013年第6期432-435,共4页Journal of Pharmaceutical Practice

摘　　要：目的优化牡丹皮提取条件，建立同时测定牡丹皮中没食子酸、芍药苷和丹皮酚3种有效成分含量的反相高效液相色谱分析方法。方法采用Agilent Plus C18柱，以乙腈-0．6％甲酸水溶液为流动相，梯度洗脱，流速1．0ml／min，检测波长254nm，进样量10μl。结果回归方程分别为：没食子酸Y=16．5980X＋4．9502，r=0．9999；芍药苷Y=1．6131X＋0．9951，r=0．9999；丹皮酚Y=5．9817X＋54．1030，r=0．9997。线性范围分别为4．0～100．1；20．6～515．0；33．3～832．0μg／ml。平均回收率分别为100．7％、99．2％、97．8％；RSD（n=9）分别为1．6％、3．0％、1．1％。结论考察了牡丹皮的提取条件，提取效果良好，分析方法准确可靠，重复性好，回收率高，适用于牡丹皮的含量测定。Objective To optimize the extraction methods and develop a HPLC-DAD assay for the determination of three effective ingredients in Moutan Coretex. Methods The analysis was performed on an Agilent ZORBAX Eclipse Plus （4.6 mm ×150 mm, 5 μm） column with acetonitrile and water （0.6% formic acid） as mobile phase at a rate of 1.0 ml/min, the detection wavelength was 254 nm, the injection volume was 10 p.1. Results The regress equation of gallic acid, paeoniflorin and paeonol were Y = 16. 598 0X ＋ 4. 950 2, r = 0. 999 9 ; Y = 1. 613 1X ＋ 0. 995 1, r = 0. 999 9 ; Y = 5.981 7X ＋ 54. 103 0, r = 0. 999 7, respectively. The average recoveries were 100.7% , 99.2% , 97.8% and RSD（n =9） were 1.6% , 3.0% , 1.1%. Conclusion The extraction effect was good and the determination assay was reliable and accurate.

关 键 词：牡丹皮 丹皮酚 芍药苷 没食子酸 高效液相色谱法 

分 类 号：R284.2[医药卫生—中药学] R917[医药卫生—中医学]