固定化乙酰胆碱脂酶的研究  

Study on Immobilization of Acetylcholinesterase

作　　者：王音[1] 

机构地区：[1]山西师范大学临汾学院自然科学系,山西临汾041000

出　　处：《山西师范大学学报（自然科学版）》2013年第4期64-68,共5页Journal of Shanxi Normal University(Natural Science Edition)

摘　　要：以戊二醛为交联剂，将乙酰胆碱脂酶交联固定到硅胶载体上，研究了影响固定化酶的主要因素．通过酶活力的测定，确定了最佳戊二醛浓度、pH值、固定化时间、酶用量、固定化酶的最适作用温度、pH值、酶的稳定性及其回收率．结果表明：戊二醛浓度为0．5％，pH值为8．0，固定3．0h，酶与载体比例为30mg／g可制备良好的功能化载体．固定化乙酰胆碱脂酶最适作用温度和pH范围分别为40℃和6．0～9．0．重复使用10次固定化酶，回收率约为70％．Acetyleholinesterase was immobilized on the silica gel using glutaraldehyde as cross linker. The concentration of glutaraldehyde, pH, time and amounts of enzyme have been optimized by means of enzymeactiv- ity. The optimum reaction temperature, pH, stability and the activity yield of the immobilized acetylcholinester- ase have been studied. The results showed that the optimum concentration of glutaraldehyde and pH value were 0.5% and 8.0 respectively, the coupling capacity of acetylcholinesterase was 30mg/g silica gel. And the opti- mal temperature and the range of pH were 40℃ and 6.0 -9.0. The activity yield of the immobilized acetylcho- linesterase was about 70% after 10 cycles reactions.

关 键 词：固定化 乙酰胆碱脂酶 硅胶 

分 类 号：Q55[生物学—生物化学]